Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.2.2 extracted from

  • Moguilevsky, N.; Garcia-Quintana, L.; Jacquet, A.; Tournay, C.; Fabry, L.; Pierard, L.; Bollen, A.
    Structural and biological properties of human recombinant myeloperoxidase produced by Chinese hamster ovary cell lines (1991), Eur. J. Biochem., 197, 605-614.
    View publication on PubMed
Search for more literature for 1.11.2.2

Activating Compound

Activating Compound Comment Organism Structure
NaCl maximal activity in the presence of 200 mM NaCl Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in CHO cell line 24.1.7 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 2 * 84000, recombinant MPO is secreted essentially as a monomeric, heme-containing, single-chain precursor of 84000 Da which exhibits peroxidase activity, gel filtration Homo sapiens
150000
-
 natural MPO, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Cl- + H2O2 + H+ Homo sapiens
-
 HClO + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant MPO has a high mannose content of 7.7%, clearly higher than the level found in the natural enzyme (4.6%) Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, carboxymethyl-Sepharose column chromatography, chelating-Sepharose column chromatography, and Sephacryl S200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 7.9 units/mg (spent culture medium, using 2-phenylenediamine as substrate, in 100 mM sodium phosphate, pH 5.0, 150 mM NaCl, at 25°C), 537 units/mg (recombinant enzyme after 67.8fold purification, using 2-phenylenediamine as substrate, in 100 mM sodium phosphate, pH 5.0, 150 mM NaCl, at 25°C), 857 units/mg (recombinant enzyme after 67.8fold purification, using O-dianisidine as substrate, in 100 mM sodium phosphate, pH 5.0, 150 mM NaCl, at 25°C), 800 units/mg (natural MPO, using O-dianisidine as substrate, in 100 mM sodium phosphate, pH 5.0, 150 mM NaCl, at 25°C) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylenediamine + H2O2
-
 Homo sapiens ?
-
 ?
Cl- + H2O2 + H+
-
 Homo sapiens HClO + H2O
-
 ?
o-dianisidine + H2O2
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 84000, recombinant MPO is secreted essentially as a monomeric, heme-containing, single-chain precursor of 84000 Da which exhibits peroxidase activity, gel filtration Homo sapiens

Synonyms

Synonyms Comment Organism
MPO
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 maximal activity around pH 5.0, recombinant and natural MPO Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme
-
 Homo sapiens