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Literature summary for 1.11.1.7 extracted from

  • Lin, M.I.; Nagata, T.; Katahira, M.
    High yield production of fungal manganese peroxidases by E. coli through soluble expression, and examination of the activities (2018), Protein Expr. Purif., 145, 45-52 .
    View publication on PubMed
Search for more literature for 1.11.1.7

Cloned(Commentary)

Cloned (Comment) Organism
gene MnP13, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein. Coexpression with disulfide bond isomerase C (DsbC) Gelatoporia subvermispora

Protein Variants

Protein Variants Comment Organism
additional information four MnPs from three MnP 88 subfamilies (extralong, long, and short MnPs) in Ceriporiopsis subvermispora are selected as model proteins for developing a universal method for MnP production, overview Gelatoporia subvermispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the activity of MnP124076 toward Mn2+ is too low to obtain reliable kinetic parameters Gelatoporia subvermispora

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, 2 Ca2+ per subunit Gelatoporia subvermispora
Fe2+ in the heme group Gelatoporia subvermispora
Mn2+ absolutely required for activity Gelatoporia subvermispora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2 Gelatoporia subvermispora
-
 2 Mn(III) + 2 H2O
-
 ?
2 Mn(II) + 2 H+ + H2O2 Gelatoporia subvermispora B
-
 2 Mn(III) + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Gelatoporia subvermispora A0A2I7M8T7
-
-
Gelatoporia subvermispora B A0A2I7M8T7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) in presence of 1 mM CaCl2 by nickel affinity chromatography, dialysis, followed by anion exchange chromatography, ultrafiltration, and gel filtration. Heme incorporation into MnP after purification and removal of excess heme by high speed centrifugation at 50000 x g Gelatoporia subvermispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2
-
 Gelatoporia subvermispora 2 Mn(III) + 2 H2O
-
 ?
2 Mn(II) + 2 H+ + H2O2
-
 Gelatoporia subvermispora B 2 Mn(III) + 2 H2O
-
 ?
2,6-dimethoxyphenol + 2 H+ + H2O2 Mn2+ is absolutely required for the activity Gelatoporia subvermispora oxidized 2,6-dimethoxyphenol + 2 H2O
-
 ?
2,6-dimethoxyphenol + 2 H+ + H2O2 Mn2+ is absolutely required for the activity Gelatoporia subvermispora B oxidized 2,6-dimethoxyphenol + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
CmMnP
-
 Gelatoporia subvermispora
MnP124076
-
 Gelatoporia subvermispora
MnP13 gene name, UniProt Gelatoporia subvermispora
More cf. EC 1.11.1.13 Gelatoporia subvermispora
short PMnP
-
 Gelatoporia subvermispora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30 assay at Gelatoporia subvermispora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 substrate 2,6-dimethoxyphenol Gelatoporia subvermispora
5
-
 substrate Mn(II) Gelatoporia subvermispora

Cofactor

Cofactor Comment Organism Structure
heme b
-
 Gelatoporia subvermispora

General Information

General Information Comment Organism
evolution MnP124076 is the only short MnP found in Ceriporiopsis subvermispora, MnP157986 is an extralong MnP and has the longest amino acid sequence. MnP50297 and MnP117436 are an extralong MnP and a long MnP, respectively, and they are the most abundantly produced MnPs in each subfamily by Ceriporiopsis subvermispora grown in aspen wood-containing medium Gelatoporia subvermispora