Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.7 extracted from

  • Almulaiky, Y.; Al-Harbi, S.
    A novel peroxidase from Arabian balsam (Commiphora gileadensis) stems Its purification, characterization and immobilization on a carboxymethylcellulose/Fe 3 O 4 magnetic hybrid material (2019), Int. J. Biol. Macromol., 133, 767-774 .
    View publication on PubMed
Search for more literature for 1.11.1.7

Application

Application Comment Organism
industry the immobilized enzyme (entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material) maintains its activity upon storage at 4 and 25°C for 8 weeks, and upon recycling for up to 15 uses. It appears to be candidate for industrial applications Commiphora gileadensis

General Stability

General Stability Organism
the immobilized enzyme entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material maintains its activity upon storage at 4 and 25°C for 8 weeks, and upon recycling for up to 15 uses Commiphora gileadensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.81
-
 H2O2 pH 7.0, temperature not specified in the publication, soluble enzyme Commiphora gileadensis
5.71
-
 H2O2 pH 7.0, temperature not specified in the publication, immobilized enzyme (entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material) Commiphora gileadensis
46.5
-
 guaiacol pH 7.0, temperature not specified in the publication, soluble enzyme Commiphora gileadensis
64.5
-
 guaiacol pH 7.0, temperature not specified in the publication, immobilized enzyme (entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material) Commiphora gileadensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Commiphora gileadensis

Organism

Organism UniProt Comment Textmining
Commiphora gileadensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, ion exchange chromatography (DEAE-Sepharose) and gel filtration (Sephcryl S-200) Commiphora gileadensis

Storage Stability

Storage Stability Organism
4°C, immobilized enzyme entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material, stable for 8 weeks Commiphora gileadensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guaiacol + H2O2
-
 Commiphora gileadensis tetraguaiacol + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Commiphora gileadensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 65 immobilized enzyme (entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material) Commiphora gileadensis
65
-
 soluble enzyme Commiphora gileadensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 75 40°C: about 65% of maximal activity, 75°C: about 75% of maximal activity, immobilized enzyme (entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material) Commiphora gileadensis
55 70 55°C: about 55% of maximal activity, 70°C: about 55% of maximal activity, soluble enzyme Commiphora gileadensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, soluble enzyme, no loss of activity Commiphora gileadensis
60
-
 30 min, immobilized enzyme entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material, no loss of activity Commiphora gileadensis
85
-
 30 min, the soluble enzyme retains approximately 13.6% of its activity, the immobilized enzyme entrapped onto a carboxymethyl cellulose/Fe3O4 magnetic hybrid material retains approximately 32% of its activity Commiphora gileadensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Commiphora gileadensis