Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, 123% of initial activity for isoform PRB-A. 112% of inital activity for isoform PRB-B | Raphanus sativus var. niger | |
Mn2+ | 5 mM, 143% of initial activity for isoform PRB-A. No activation of isoform PRB-B | Raphanus sativus var. niger | |
Ni2+ | 5 mM, 139% of initial activity for isoform PRB-A. No activation of isoform PRB-B | Raphanus sativus var. niger | |
Zn2+ | 5 mM, 143% of initial activity for isoform PRB-A. 121% of inital activity for isoform PRB-B | Raphanus sativus var. niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Raphanus sativus var. niger | |
citrate | - |
Raphanus sativus var. niger | |
EDTA | - |
Raphanus sativus var. niger | |
guanidine hydrochloride | less than 10% activity at 1M guanidine hydrochloride | Raphanus sativus var. niger | |
SDS | about 75% loss of activity at 2% W/V SDS | Raphanus sativus var. niger | |
Urea | loss of more than 50% of its activity at 1M urea | Raphanus sativus var. niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
H2O2 | isoform PRB-A, pH 7, pH 7, 23°C | Raphanus sativus var. niger | |
0.067 | - |
H2O2 | isoform PRB-B, pH 7, pH 7, 23°C | Raphanus sativus var. niger | |
10.63 | - |
4-aminoantipyrine | isoform PRB-A, pH 7, pH 7, 23°C | Raphanus sativus var. niger | |
15.38 | - |
4-aminoantipyrine | isoform PRB-B, pH 7, pH 7, 23°C | Raphanus sativus var. niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus var. niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protein extraction by a combination of freeze-thawing and grinding, ultrafiltration and diafiltration, ammonium sulfate precipitation, DEAE anion-exchange chromatography, and concanavalin A affinity chromatography | Raphanus sativus var. niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Raphanus sativus var. niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
166.7 | - |
pH 7, 23°C, assay mixture contains 18 mM 4-aminoantipyrine, 0.2 mM H2O2, and 85 mM phenol | Raphanus sativus var. niger |
Storage Stability | Organism |
---|---|
37°C, stable for at least 120 days | Raphanus sativus var. niger |
4°C, stable for at least 120 days | Raphanus sativus var. niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminoantipyrine + phenol + H2O2 | - |
Raphanus sativus var. niger | quinoneimine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000, isoform BRP-A, 1 * 97000, isoform BRP-B, SDS-PAGE | Raphanus sativus var. niger |
Synonyms | Comment | Organism |
---|---|---|
black radish peroxidase A | - |
Raphanus sativus var. niger |
black radish peroxidase B | - |
Raphanus sativus var. niger |
BRP-A | - |
Raphanus sativus var. niger |
BRP-B | - |
Raphanus sativus var. niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
both isoforms BRP-A and BRP-B | Raphanus sativus var. niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
isoform BRP-A | Raphanus sativus var. niger |
6 | - |
isoform BRP-B | Raphanus sativus var. niger |