Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
C13N | the mutant shows reduced activity compared to the wild type enzyme | Enterococcus faecalis |
C13N/C66V | the mutant shows reduced activity compared to the wild type enzyme | Enterococcus faecalis |
C66V | the mutant shows reduced activity compared to the wild type enzyme | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H2O2 + H+ | Enterococcus faecalis | - |
NAD+ + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 200 gel filtration | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H2O2 + H+ | - |
Enterococcus faecalis | NAD+ + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AhpC | - |
Enterococcus faecalis |
alkylhydroperoxide reductase | - |
Enterococcus faecalis |