Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.26 extracted from

  • Toh, Y.K.; Shin, J.; Balakrishna, A.M.; Kamariah, N.; Grueber, A.; Eisenhaber, F.; Eisenhaber, B.; Grueber, G.
    Effect of the additional cysteine 503 of vancomycin-resistant Enterococcus faecalis (V583) alkylhydroperoxide reductase subunit F (AhpF) and the mechanism of AhpF and subunit C assembling (2019), Free Radic. Biol. Med., 138, 10-22 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Enterococcus faecalis

Protein Variants

Protein Variants Comment Organism
C13N the mutant shows reduced activity compared to the wild type enzyme Enterococcus faecalis
C13N/C66V the mutant shows reduced activity compared to the wild type enzyme Enterococcus faecalis
C66V the mutant shows reduced activity compared to the wild type enzyme Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H2O2 + H+ Enterococcus faecalis
-
NAD+ + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Superdex 200 gel filtration Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H2O2 + H+
-
Enterococcus faecalis NAD+ + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
AhpC
-
Enterococcus faecalis
alkylhydroperoxide reductase
-
Enterococcus faecalis