Crystallization (Comment) | Organism |
---|---|
hanging drop method | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants | Comment | Organism |
---|---|---|
C165S | kcat/Km for H2O2 is 192fold lower than wild-type value | Salmonella enterica subsp. enterica serovar Typhimurium |
C165S | the mutant shows 0.52% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
E49Q | kcat/Km for H2O2 is 47619fold lower than wild-type value. Mutations destabilizes decamers in the reduced form | Salmonella enterica subsp. enterica serovar Typhimurium |
E49Q | the mutation lowers catalytic efficiency with hydrogen peroxide by 4-5 orders of magnitude, but does not affect reactivity toward their reductant, AhpF. The mutant shows 0.0021% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
R119A | kcat/Km for H2O2 is 5882fold lower than wild-type value. Mutations destabilizes decamers in the reduced form | Salmonella enterica subsp. enterica serovar Typhimurium |
R119A | the mutation lowers catalytic efficiency with hydrogen peroxide by 4-5 orders of magnitude, but does not affect reactivity toward their reductant, AhpF. The mutant shows 0.017% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
T43A | kcat/Km for H2O2 is 434fold lower than wild-type value | Salmonella enterica subsp. enterica serovar Typhimurium |
T43A | the mutant exhibits stabilized decamers and is more efficiently reduced by AhpF than wild type enzyme. The mutant shows 0.23% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
T43S | kcat/Km for H2O2 is 2fold lower than wild-type value. Mutation stabilizes oxidized decameric form | Salmonella enterica subsp. enterica serovar Typhimurium |
T43S | the mutant exhibits stabilized decamers and is more efficiently reduced by AhpF than wild type enzyme. The mutant shows 48% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
T43V | kcat/Km for H2O2 is 26315fold lower than wild-type value. Mutations destabilizes decamers in the reduced form. Mutation stabilizes oxidized decameric form | Salmonella enterica subsp. enterica serovar Typhimurium |
T43V | the mutation lowers catalytic efficiency with hydrogen peroxide by 4-5 orders of magnitude, but does not affect reactivity toward their reductant, AhpF. The mutant shows 0.0038% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
T77I | kcat/Km for H2O2 is 139fold lower than wild-type value | Salmonella enterica subsp. enterica serovar Typhimurium |
T77I | the mutant shows 0.72% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
T77V | kcat/Km for H2O2 is 83% comared to wild-type value. Mutation stabilizes reduced and oxidized decameric form | Salmonella enterica subsp. enterica serovar Typhimurium |
T77V | the mutant exhibits stabilized decamers and is more efficiently reduced by AhpF than wild type enzyme. The mutant shows 120% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
W169F | kcat/Km for H2O2 is 151fold lower than wild-type value | Salmonella enterica subsp. enterica serovar Typhimurium |
W169F | the mutant shows 0.66% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
W81F | kcat/Km for H2O2 is 6.7fold lower than wild-type value | Salmonella enterica subsp. enterica serovar Typhimurium |
W81F | the mutant shows 15% activity with H2O2 compared to the wild type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00039 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T43S | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.00039 | - |
H2O2 | mutant enzyme T43S, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.00084 | - |
H2O2 | pH 8.5, 30°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.00084 | - |
H2O2 | wild type enzyme, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0011 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T77V | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0011 | - |
H2O2 | mutant enzyme T77V, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0015 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme C165S | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0015 | - |
H2O2 | mutant enzyme C165S, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0027 | - |
H2O2 | pH 8.0, 30°C, mutant enzyme W81F | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0027 | - |
H2O2 | mutant enzyme W81F, at pH 8.0, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.014 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T77I | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.014 | - |
H2O2 | mutant enzyme T77I, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.031 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme W169F | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.031 | - |
H2O2 | mutant enzyme W169F, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.034 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T43A | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.034 | - |
H2O2 | mutant enzyme T43A, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.74 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme E49Q | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.74 | - |
H2O2 | mutant enzyme E49Q, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.5 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme R119A | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.5 | - |
H2O2 | mutant enzyme R119A, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.6 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T43V | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.6 | - |
H2O2 | mutant enzyme T43V, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H2O2 + H+ | Salmonella enterica subsp. enterica serovar Typhimurium | - |
NAD+ + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | P0A251 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | P0A251 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H2O2 | - |
Salmonella enterica subsp. enterica serovar Typhimurium | NAD+ + H2O | - |
? | |
NADH + H2O2 | - |
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 | NAD+ + H2O | - |
? | |
NADH + H2O2 + H+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | NAD+ + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AhpC | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme C165S | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.4 | - |
H2O2 | mutant enzyme C165S, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.73 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme E49Q | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.73 | - |
H2O2 | mutant enzyme E49Q, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.8 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T43V | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.8 | - |
H2O2 | mutant enzyme T43V, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
3.8 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T43A | Salmonella enterica subsp. enterica serovar Typhimurium | |
3.8 | - |
H2O2 | mutant enzyme T43A, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.8 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T77I | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.8 | - |
H2O2 | mutant enzyme T77I, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
8.9 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T43S | Salmonella enterica subsp. enterica serovar Typhimurium | |
8.9 | - |
H2O2 | mutant enzyme T43S, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
9.6 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme W169F | Salmonella enterica subsp. enterica serovar Typhimurium | |
9.6 | - |
H2O2 | mutant enzyme W169F, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
11.7 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme R119A | Salmonella enterica subsp. enterica serovar Typhimurium | |
11.7 | - |
H2O2 | mutant enzyme R119A, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
19 | - |
H2O2 | pH 8.0, 30°C, mutant enzyme W81F | Salmonella enterica subsp. enterica serovar Typhimurium | |
19 | - |
H2O2 | mutant enzyme W81F, at pH 8.0, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
40 | - |
H2O2 | pH 8.5, 30°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
40 | - |
H2O2 | wild type enzyme, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
61 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T77V | Salmonella enterica subsp. enterica serovar Typhimurium | |
61 | - |
H2O2 | mutant enzyme T77V, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.99 | - |
H2O2 | mutant enzyme E49Q, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.8 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T43V | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.8 | - |
H2O2 | mutant enzyme T43V, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
8 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme R119A | Salmonella enterica subsp. enterica serovar Typhimurium | |
8 | - |
H2O2 | mutant enzyme R119A, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
99 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme E49Q | Salmonella enterica subsp. enterica serovar Typhimurium | |
110 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T43A | Salmonella enterica subsp. enterica serovar Typhimurium | |
110 | - |
H2O2 | mutant enzyme T43A, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
270 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme C165S | Salmonella enterica subsp. enterica serovar Typhimurium | |
270 | - |
H2O2 | mutant enzyme C165S, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
310 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme W169F | Salmonella enterica subsp. enterica serovar Typhimurium | |
310 | - |
H2O2 | mutant enzyme W169F, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
340 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T77I | Salmonella enterica subsp. enterica serovar Typhimurium | |
340 | - |
H2O2 | mutant enzyme T77I, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
7200 | - |
H2O2 | pH 8.0, 30°C, mutant enzyme W81F | Salmonella enterica subsp. enterica serovar Typhimurium | |
7200 | - |
H2O2 | mutant enzyme W81F, at pH 8.0, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
23000 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T43S | Salmonella enterica subsp. enterica serovar Typhimurium | |
23000 | - |
H2O2 | mutant enzyme T43S, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
47000 | - |
H2O2 | pH 8.5, 30°C, wild-type enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
47000 | - |
H2O2 | wild type enzyme, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
55000 | - |
H2O2 | pH 8.5, 30°C, mutant enzyme T77V | Salmonella enterica subsp. enterica serovar Typhimurium | |
55000 | - |
H2O2 | mutant enzyme T77V, at pH 8.5, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |