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Literature summary for 1.11.1.26 extracted from

  • Nelson, K.; Perkins, A.; Van Swearingen, A.; Hartman, S.; Brereton, A.; Parsonage, D.; Salsbury, F.; Karplus, P.; Poole, L.
    Experimentally dissecting the origins of peroxiredoxin catalysis (2018), Antioxid. Redox Signal., 28, 521-536 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
C165S kcat/Km for H2O2 is 192fold lower than wild-type value Salmonella enterica subsp. enterica serovar Typhimurium
C165S the mutant shows 0.52% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
E49Q kcat/Km for H2O2 is 47619fold lower than wild-type value. Mutations destabilizes decamers in the reduced form Salmonella enterica subsp. enterica serovar Typhimurium
E49Q the mutation lowers catalytic efficiency with hydrogen peroxide by 4-5 orders of magnitude, but does not affect reactivity toward their reductant, AhpF. The mutant shows 0.0021% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
R119A kcat/Km for H2O2 is 5882fold lower than wild-type value. Mutations destabilizes decamers in the reduced form Salmonella enterica subsp. enterica serovar Typhimurium
R119A the mutation lowers catalytic efficiency with hydrogen peroxide by 4-5 orders of magnitude, but does not affect reactivity toward their reductant, AhpF. The mutant shows 0.017% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
T43A kcat/Km for H2O2 is 434fold lower than wild-type value Salmonella enterica subsp. enterica serovar Typhimurium
T43A the mutant exhibits stabilized decamers and is more efficiently reduced by AhpF than wild type enzyme. The mutant shows 0.23% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
T43S kcat/Km for H2O2 is 2fold lower than wild-type value. Mutation stabilizes oxidized decameric form Salmonella enterica subsp. enterica serovar Typhimurium
T43S the mutant exhibits stabilized decamers and is more efficiently reduced by AhpF than wild type enzyme. The mutant shows 48% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
T43V kcat/Km for H2O2 is 26315fold lower than wild-type value. Mutations destabilizes decamers in the reduced form. Mutation stabilizes oxidized decameric form Salmonella enterica subsp. enterica serovar Typhimurium
T43V the mutation lowers catalytic efficiency with hydrogen peroxide by 4-5 orders of magnitude, but does not affect reactivity toward their reductant, AhpF. The mutant shows 0.0038% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
T77I kcat/Km for H2O2 is 139fold lower than wild-type value Salmonella enterica subsp. enterica serovar Typhimurium
T77I the mutant shows 0.72% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
T77V kcat/Km for H2O2 is 83% comared to wild-type value. Mutation stabilizes reduced and oxidized decameric form Salmonella enterica subsp. enterica serovar Typhimurium
T77V the mutant exhibits stabilized decamers and is more efficiently reduced by AhpF than wild type enzyme. The mutant shows 120% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
W169F kcat/Km for H2O2 is 151fold lower than wild-type value Salmonella enterica subsp. enterica serovar Typhimurium
W169F the mutant shows 0.66% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
W81F kcat/Km for H2O2 is 6.7fold lower than wild-type value Salmonella enterica subsp. enterica serovar Typhimurium
W81F the mutant shows 15% activity with H2O2 compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00039
-
H2O2 pH 8.5, 30°C, mutant enzyme T43S Salmonella enterica subsp. enterica serovar Typhimurium
0.00039
-
H2O2 mutant enzyme T43S, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.00084
-
H2O2 pH 8.5, 30°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
0.00084
-
H2O2 wild type enzyme, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.0011
-
H2O2 pH 8.5, 30°C, mutant enzyme T77V Salmonella enterica subsp. enterica serovar Typhimurium
0.0011
-
H2O2 mutant enzyme T77V, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.0015
-
H2O2 pH 8.5, 30°C, mutant enzyme C165S Salmonella enterica subsp. enterica serovar Typhimurium
0.0015
-
H2O2 mutant enzyme C165S, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.0027
-
H2O2 pH 8.0, 30°C, mutant enzyme W81F Salmonella enterica subsp. enterica serovar Typhimurium
0.0027
-
H2O2 mutant enzyme W81F, at pH 8.0, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.014
-
H2O2 pH 8.5, 30°C, mutant enzyme T77I Salmonella enterica subsp. enterica serovar Typhimurium
0.014
-
H2O2 mutant enzyme T77I, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.031
-
H2O2 pH 8.5, 30°C, mutant enzyme W169F Salmonella enterica subsp. enterica serovar Typhimurium
0.031
-
H2O2 mutant enzyme W169F, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.034
-
H2O2 pH 8.5, 30°C, mutant enzyme T43A Salmonella enterica subsp. enterica serovar Typhimurium
0.034
-
H2O2 mutant enzyme T43A, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.74
-
H2O2 pH 8.5, 30°C, mutant enzyme E49Q Salmonella enterica subsp. enterica serovar Typhimurium
0.74
-
H2O2 mutant enzyme E49Q, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
1.5
-
H2O2 pH 8.5, 30°C, mutant enzyme R119A Salmonella enterica subsp. enterica serovar Typhimurium
1.5
-
H2O2 mutant enzyme R119A, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
1.6
-
H2O2 pH 8.5, 30°C, mutant enzyme T43V Salmonella enterica subsp. enterica serovar Typhimurium
1.6
-
H2O2 mutant enzyme T43V, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H2O2 + H+ Salmonella enterica subsp. enterica serovar Typhimurium
-
NAD+ + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium P0A251
-
-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 P0A251
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H2O2
-
Salmonella enterica subsp. enterica serovar Typhimurium NAD+ + H2O
-
?
NADH + H2O2
-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 NAD+ + H2O
-
?
NADH + H2O2 + H+
-
Salmonella enterica subsp. enterica serovar Typhimurium NAD+ + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
AhpC
-
Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
H2O2 pH 8.5, 30°C, mutant enzyme C165S Salmonella enterica subsp. enterica serovar Typhimurium
0.4
-
H2O2 mutant enzyme C165S, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
0.73
-
H2O2 pH 8.5, 30°C, mutant enzyme E49Q Salmonella enterica subsp. enterica serovar Typhimurium
0.73
-
H2O2 mutant enzyme E49Q, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.8
-
H2O2 pH 8.5, 30°C, mutant enzyme T43V Salmonella enterica subsp. enterica serovar Typhimurium
2.8
-
H2O2 mutant enzyme T43V, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
3.8
-
H2O2 pH 8.5, 30°C, mutant enzyme T43A Salmonella enterica subsp. enterica serovar Typhimurium
3.8
-
H2O2 mutant enzyme T43A, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
4.8
-
H2O2 pH 8.5, 30°C, mutant enzyme T77I Salmonella enterica subsp. enterica serovar Typhimurium
4.8
-
H2O2 mutant enzyme T77I, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
8.9
-
H2O2 pH 8.5, 30°C, mutant enzyme T43S Salmonella enterica subsp. enterica serovar Typhimurium
8.9
-
H2O2 mutant enzyme T43S, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
9.6
-
H2O2 pH 8.5, 30°C, mutant enzyme W169F Salmonella enterica subsp. enterica serovar Typhimurium
9.6
-
H2O2 mutant enzyme W169F, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
11.7
-
H2O2 pH 8.5, 30°C, mutant enzyme R119A Salmonella enterica subsp. enterica serovar Typhimurium
11.7
-
H2O2 mutant enzyme R119A, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
19
-
H2O2 pH 8.0, 30°C, mutant enzyme W81F Salmonella enterica subsp. enterica serovar Typhimurium
19
-
H2O2 mutant enzyme W81F, at pH 8.0, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
40
-
H2O2 pH 8.5, 30°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
40
-
H2O2 wild type enzyme, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
61
-
H2O2 pH 8.5, 30°C, mutant enzyme T77V Salmonella enterica subsp. enterica serovar Typhimurium
61
-
H2O2 mutant enzyme T77V, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.99
-
H2O2 mutant enzyme E49Q, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
1.8
-
H2O2 pH 8.5, 30°C, mutant enzyme T43V Salmonella enterica subsp. enterica serovar Typhimurium
1.8
-
H2O2 mutant enzyme T43V, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
8
-
H2O2 pH 8.5, 30°C, mutant enzyme R119A Salmonella enterica subsp. enterica serovar Typhimurium
8
-
H2O2 mutant enzyme R119A, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
99
-
H2O2 pH 8.5, 30°C, mutant enzyme E49Q Salmonella enterica subsp. enterica serovar Typhimurium
110
-
H2O2 pH 8.5, 30°C, mutant enzyme T43A Salmonella enterica subsp. enterica serovar Typhimurium
110
-
H2O2 mutant enzyme T43A, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
270
-
H2O2 pH 8.5, 30°C, mutant enzyme C165S Salmonella enterica subsp. enterica serovar Typhimurium
270
-
H2O2 mutant enzyme C165S, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
310
-
H2O2 pH 8.5, 30°C, mutant enzyme W169F Salmonella enterica subsp. enterica serovar Typhimurium
310
-
H2O2 mutant enzyme W169F, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
340
-
H2O2 pH 8.5, 30°C, mutant enzyme T77I Salmonella enterica subsp. enterica serovar Typhimurium
340
-
H2O2 mutant enzyme T77I, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
7200
-
H2O2 pH 8.0, 30°C, mutant enzyme W81F Salmonella enterica subsp. enterica serovar Typhimurium
7200
-
H2O2 mutant enzyme W81F, at pH 8.0, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
23000
-
H2O2 pH 8.5, 30°C, mutant enzyme T43S Salmonella enterica subsp. enterica serovar Typhimurium
23000
-
H2O2 mutant enzyme T43S, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
47000
-
H2O2 pH 8.5, 30°C, wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
47000
-
H2O2 wild type enzyme, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
55000
-
H2O2 pH 8.5, 30°C, mutant enzyme T77V Salmonella enterica subsp. enterica serovar Typhimurium
55000
-
H2O2 mutant enzyme T77V, at pH 8.5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium