Cloned (Comment) | Organism |
---|---|
- |
Corallina pilulifera |
Crystallization (Comment) | Organism |
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sitting drop vapour diffusion method, two crystal forms are obtained, one hexagonal form using ammonium phosphate as precipitant (form 1) and a second cubic vanadium bound form (form 2). The best crystals of the cubic form 2 containing vanadate are only obtained with the wild type enzyme. The optimised conditions use an initial protein concentration of 18 mg/ml in 50 mM Tris-H2SO4, 0.4 M KBr, 1 mM Na3VO4, 20% (w/v) polyethylene glycol 6000. For the wild type enzyme crystals grown in form 2, a mother liquor substituting the precipitant with 25% polyethylene glycol 400 and 25% polyethylene glycol 6000 is used | Corallina pilulifera |
Protein Variants | Comment | Organism |
---|---|---|
R397W | the mutant shows a different behaviour on bromide binding compared to the wild type enzyme, (the bromide is missing the hydrogen-binding interaction that held it in the active site of the wild type enzyme) | Corallina pilulifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corallina pilulifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corallina pilulifera |
Synonyms | Comment | Organism |
---|---|---|
vanadium containing bromoperoxidase | - |
Corallina pilulifera |