Application | Comment | Organism |
---|---|---|
biofuel production | bioethanol production, the enzymes laccase and manganese peroxidase from Klebsiella pneumoniae are employed for ethanol production from rice and wheat bran biomass which shows 39.29% improved production compared to control, evaluation | Klebsiella pneumoniae |
synthesis | the enzymes laccase and manganese peroxidase from Klebsiella pneumoniae are employed for ethanol production from rice and wheat bran biomass which shows 39.29% improved production compared to control, evaluation | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | MnP production from NITW715076_2, NITW715076_1, and NITW715076 isolates | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | at higher concentration | Klebsiella pneumoniae | |
L-cysteine | - |
Klebsiella pneumoniae | |
SDS | - |
Klebsiella pneumoniae | |
Sodium azide | - |
Klebsiella pneumoniae | |
sodium sulfite | - |
Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.173 | - |
Mn(II) | strain NITW715076_2, pH 5.0, 50°C | Klebsiella pneumoniae | |
0.197 | - |
Mn(II) | strain NITW715076, pH 5.0, 60°C | Klebsiella pneumoniae | |
0.904 | - |
Mn(II) | strain NITW715076_1, pH 5.0, 70°C | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | Klebsiella pneumoniae | - |
2 Mn(III) + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 2.73fold from strain NITW715076, 1.6fold from strain NITW715076_1, and 2.85fold from strain NITW715076_2, by ammonium sulfate fractionation, dialysis, and anion exchange chromatography | Klebsiella pneumoniae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the optimum temperatures for MnP production from NITW715076_2, NITW715076_1 and NITW715076 isolates are 50°C, 70°C, and 60°C, respectively. Fermentation is carried out using hydrolysate obtained after pre-treatment. The hydrolysate is mixed with 3 ml of pure MnP and 0.1% w/v Saccharomyces cerevisiae for 384 h at 35°C. The optimized conditions achieved for enzyme production are pH 6.5, temperature 35°C, agitation speed 130 rpm, carbon source sucrose (1%), nitrogen source yeast extract (0.5%) and inoculum volume 4 ml/100 ml | Klebsiella pneumoniae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
maximum MnP activity for strain NITW715076_2 is 92.77 U/ml at 50°C. For strains NITW715076_1 and NITW715076, the MnP activity is 145.65 U/ml1 at 70°C and 107.24 U/ml1 at 60°C, respectively | Klebsiella pneumoniae |
84.08 | - |
purified enzyme from strain NITW715076_2, pH 5.0, 60°C | Klebsiella pneumoniae |
101.85 | - |
purified enzyme from strain NITW715076_1, pH 5.0, 60°C | Klebsiella pneumoniae |
241.18 | - |
purified enzyme from strain NITW715076, pH 5.0, 60°C | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | - |
Klebsiella pneumoniae | 2 Mn(III) + 2 H2O | - |
? | |
phenol red + H2O2 | - |
Klebsiella pneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
MnP | - |
Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | maximum MnP activity for strain NITW715076_2 is observed at 50°C, for strains NITW715076_1 and NITW715076 at 70°C and 60°C, respectively | Klebsiella pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | activity range, profile overview | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | activity range, profile overview | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | stable up to, loss of 45-50% activity above | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Klebsiella pneumoniae |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme MnP generates Mn3+ (act as a diffusible charge transfer mediators) which can oxidize a large amount of phenolic substrates such as simple phenols, amines, dyes as well as phenolic lignin model compounds. In contrast to laccase, MnP is not capable of oxidizing the more recalcitrant nonphenolic compounds | Klebsiella pneumoniae |