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Literature summary for 1.11.1.13 extracted from

  • Gaur, N.; Narasimhulu, K.; Pydisetty, Y.
    Biochemical and kinetic characterization of laccase and manganese peroxidase from novel Klebsiella pneumoniae strains and their application in bioethanol production (2018), RSC Adv., 8, 15044-15055 .
No PubMed abstract available

Application

Application Comment Organism
biofuel production bioethanol production, the enzymes laccase and manganese peroxidase from Klebsiella pneumoniae are employed for ethanol production from rice and wheat bran biomass which shows 39.29% improved production compared to control, evaluation Klebsiella pneumoniae
synthesis the enzymes laccase and manganese peroxidase from Klebsiella pneumoniae are employed for ethanol production from rice and wheat bran biomass which shows 39.29% improved production compared to control, evaluation Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information MnP production from NITW715076_2, NITW715076_1, and NITW715076 isolates Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
EDTA at higher concentration Klebsiella pneumoniae
L-cysteine
-
Klebsiella pneumoniae
SDS
-
Klebsiella pneumoniae
Sodium azide
-
Klebsiella pneumoniae
sodium sulfite
-
Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.173
-
Mn(II) strain NITW715076_2, pH 5.0, 50°C Klebsiella pneumoniae
0.197
-
Mn(II) strain NITW715076, pH 5.0, 60°C Klebsiella pneumoniae
0.904
-
Mn(II) strain NITW715076_1, pH 5.0, 70°C Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2 Klebsiella pneumoniae
-
2 Mn(III) + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 2.73fold from strain NITW715076, 1.6fold from strain NITW715076_1, and 2.85fold from strain NITW715076_2, by ammonium sulfate fractionation, dialysis, and anion exchange chromatography Klebsiella pneumoniae

Source Tissue

Source Tissue Comment Organism Textmining
additional information the optimum temperatures for MnP production from NITW715076_2, NITW715076_1 and NITW715076 isolates are 50°C, 70°C, and 60°C, respectively. Fermentation is carried out using hydrolysate obtained after pre-treatment. The hydrolysate is mixed with 3 ml of pure MnP and 0.1% w/v Saccharomyces cerevisiae for 384 h at 35°C. The optimized conditions achieved for enzyme production are pH 6.5, temperature 35°C, agitation speed 130 rpm, carbon source sucrose (1%), nitrogen source yeast extract (0.5%) and inoculum volume 4 ml/100 ml Klebsiella pneumoniae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
maximum MnP activity for strain NITW715076_2 is 92.77 U/ml at 50°C. For strains NITW715076_1 and NITW715076, the MnP activity is 145.65 U/ml1 at 70°C and 107.24 U/ml1 at 60°C, respectively Klebsiella pneumoniae
84.08
-
purified enzyme from strain NITW715076_2, pH 5.0, 60°C Klebsiella pneumoniae
101.85
-
purified enzyme from strain NITW715076_1, pH 5.0, 60°C Klebsiella pneumoniae
241.18
-
purified enzyme from strain NITW715076, pH 5.0, 60°C Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2
-
Klebsiella pneumoniae 2 Mn(III) + 2 H2O
-
?
phenol red + H2O2
-
Klebsiella pneumoniae ?
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
MnP
-
Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 70 maximum MnP activity for strain NITW715076_2 is observed at 50°C, for strains NITW715076_1 and NITW715076 at 70°C and 60°C, respectively Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity range, profile overview Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
3 8 activity range, profile overview Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5 stable up to, loss of 45-50% activity above Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
heme
-
Klebsiella pneumoniae

General Information

General Information Comment Organism
physiological function enzyme MnP generates Mn3+ (act as a diffusible charge transfer mediators) which can oxidize a large amount of phenolic substrates such as simple phenols, amines, dyes as well as phenolic lignin model compounds. In contrast to laccase, MnP is not capable of oxidizing the more recalcitrant nonphenolic compounds Klebsiella pneumoniae