Cloned (Comment) | Organism |
---|---|
gene Il-MnP6, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) in inclusion bodies | Irpex lacteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 85% inhibition at 0.1 mM, complete inhibition at 1 mM | Irpex lacteus | |
Al3+ | 8% inhibition at 1 mM, 78% inhibition at 10 mM | Irpex lacteus | |
Ca2+ | 10% inhibition at 10 mM | Irpex lacteus | |
Cd2+ | 49% inhibition at 10 mM | Irpex lacteus | |
Cu2+ | 50% inhibition at 10 mM | Irpex lacteus | |
DTT | 75% inhibition at 0.1 mM, complete inhibition at 1 mM | Irpex lacteus | |
EDTA | 92% inhibition at 1 mM, 96% inhibition at 10 mM | Irpex lacteus | |
Fe2+ | 81% inhibition at 0.1 mM, complete inhibition at 1 mM | Irpex lacteus | |
Fe3+ | 64% inhibition at 1 mM, complete inhibition at 10 mM | Irpex lacteus | |
H2O2 | the enzyme activity is reduced by 10% as the H2O2 concentration increases from 0.1 to 4 mM | Irpex lacteus | |
Ni2+ | 8% inhibition at 10 mM | Irpex lacteus | |
Thiourea | 81% inhibition at 10 mM | Irpex lacteus | |
Zn2+ | 15% inhibition at 10 mM | Irpex lacteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
guaiacol | pH 4.0, 45°C, recombinant His6-tagged enzyme | Irpex lacteus | |
0.0044 | - |
Reactive Black 5 | pH 3.0, 35°C, recombinant His6-tagged enzyme | Irpex lacteus | |
0.053 | - |
2,6-dimethoxyphenol | pH 4.5, 55°C, recombinant His6-tagged enzyme | Irpex lacteus | |
0.081 | - |
Reactive Blue 19 | pH 3.0, 35°C, recombinant His6-tagged enzyme | Irpex lacteus | |
0.083 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 45°C, recombinant His6-tagged enzyme | Irpex lacteus | |
0.129 | - |
Mn(II) | pH 4.5, 30°C, recombinant His6-tagged enzyme | Irpex lacteus | |
0.194 | - |
H2O2 | pH 4.5, 30°C, recombinant His6-tagged enzyme | Irpex lacteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 15% activation at 10 mM | Irpex lacteus | |
Co2+ | 11% activation at 10 mM | Irpex lacteus | |
additional information | poor effect on enzyme activity by Mn2+, Na+, and Mg2+ at 0.1-10 mM | Irpex lacteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | Irpex lacteus | - |
2 Mn(III) + 2 H2O | - |
? | |
2 Mn(II) + 2 H+ + H2O2 | Irpex lacteus F17 | - |
2 Mn(III) + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Irpex lacteus | A0A2P1C6N7 | - |
- |
Irpex lacteus F17 | A0A2P1C6N7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant refolded His6-tagged enzyme expressed from Escherichia coli strain Rosetta (DE3) by dialysis and nickel affinity chromatography | Irpex lacteus |
Renatured (Comment) | Organism |
---|---|
recombinant His6-tagged isozyme MnP6 from Escherichia coli strain Rosetta(DE3) inclusion bodies, refolding conditions: 1.5 M urea, 150 mM CaCl2, 0.025 mM hemin, 10% glycerol, 0.5 mM GSSG, 0.05 mM MnSO4, 20 mM KCl, and 50 mM Tris-HCl, pH 8.5 | Irpex lacteus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.8 | - |
pH 4.5, 30°C, purified recombinant refolded His6-tagged enzyme | Irpex lacteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | - |
Irpex lacteus | 2 Mn(III) + 2 H2O | - |
? | |
2 Mn(II) + 2 H+ + H2O2 | - |
Irpex lacteus F17 | 2 Mn(III) + 2 H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H+ + H2O2 | ABTS | Irpex lacteus | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H+ + H2O2 | ABTS | Irpex lacteus F17 | oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O | - |
? | |
2,6-dimethoxyphenol + 2 H+ + H2O2 | - |
Irpex lacteus | oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? | |
2,6-dimethoxyphenol + 2 H+ + H2O2 | - |
Irpex lacteus F17 | oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? | |
guaiacol + 2 H+ + H2O2 | - |
Irpex lacteus | oxidized guaiacol + 2 H2O | - |
? | |
guaiacol + 2 H+ + H2O2 | - |
Irpex lacteus F17 | oxidized guaiacol + 2 H2O | - |
? | |
additional information | no activity with veratryl alcohol (VA) | Irpex lacteus | ? | - |
- |
|
additional information | no activity with veratryl alcohol (VA) | Irpex lacteus F17 | ? | - |
- |
|
Reactive Black 5 + 2 H+ + H2O2 | dye decolorization | Irpex lacteus | Reactive Black 5 + 2 H2O | - |
? | |
Reactive Blue 19 + 2 H+ + H2O2 | dye decolorization | Irpex lacteus | oxidized Reactive Blue 19 + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44600, recombinant refolded His6-tagged enzyme, SDS-PAGE, x * 38200, about, MnP6, sequence calculation | Irpex lacteus |
More | enzyme secondary structure analysis overview | Irpex lacteus |
Synonyms | Comment | Organism |
---|---|---|
Il-MnP6 | - |
Irpex lacteus |
MnP | - |
Irpex lacteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Irpex lacteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified recombinant refolded His6-tagged enzyme retaining over 90% of its enzymatic activity below 45°C for 12 h | Irpex lacteus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
Reactive Black 5 | pH 3.0, 35°C, recombinant His6-tagged enzyme | Irpex lacteus | |
7.3 | - |
Reactive Blue 19 | pH 3.0, 35°C, recombinant His6-tagged enzyme | Irpex lacteus | |
184 | - |
guaiacol | pH 4.0, 45°C, recombinant His6-tagged enzyme | Irpex lacteus | |
245 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 45°C, recombinant His6-tagged enzyme | Irpex lacteus | |
369 | - |
Mn(II) | pH 4.5, 30°C, recombinant His6-tagged enzyme | Irpex lacteus | |
879 | - |
H2O2 | pH 4.5, 30°C, recombinant His6-tagged enzyme | Irpex lacteus | |
989 | - |
2,6-dimethoxyphenol | pH 4.5, 55°C, recombinant His6-tagged enzyme | Irpex lacteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
optimum peak 1 | Irpex lacteus |
7 | - |
optimum peak 2 | Irpex lacteus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7.5 | purified recombinant refolded His6-tagged enzyme retaining 75% of maximal activity within this range | Irpex lacteus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Irpex lacteus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the ligninolytic peroxidase gene family, 13 MnP genes and five dye-decolorizing peroxidase (DyP) genes are present in the Irpex lacteus strain F17 genome, they represent two different families of heme peroxidases and are unrelated to the fungal class II peroxidases | Irpex lacteus |
physiological function | the manganese peroxidase of Irpex lacteus strain 17 is able to degrade recalcitrant aromatic pollutants | Irpex lacteus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.67 | - |
Reactive Black 5 | pH 3.0, 35°C, recombinant His6-tagged enzyme | Irpex lacteus | |
90.12 | - |
Reactive Blue 19 | pH 3.0, 35°C, recombinant His6-tagged enzyme | Irpex lacteus | |
2860.5 | - |
Mn(II) | pH 4.5, 30°C, recombinant His6-tagged enzyme | Irpex lacteus | |
2951.8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 45°C, recombinant His6-tagged enzyme | Irpex lacteus | |
4530.9 | - |
H2O2 | pH 4.5, 30°C, recombinant His6-tagged enzyme | Irpex lacteus | |
18660.4 | - |
2,6-dimethoxyphenol | pH 4.5, 55°C, recombinant His6-tagged enzyme | Irpex lacteus | |
70769 | - |
guaiacol | pH 4.0, 45°C, recombinant His6-tagged enzyme | Irpex lacteus |