Application | Comment | Organism |
---|---|---|
environmental protection | - |
Phanerodontia chrysosporium |
environmental protection | degradation of recalcitrant high-molecular-mass compounds, such as nylon and melanin, degradation of xenobiotic compounds, bioremediation, decolorization of wastewater | Deuteromycotina sp. |
paper production | - |
Phanerodontia chrysosporium |
paper production | biobleaching of kraft pulp | Deuteromycotina sp. |
Cloned (Comment) | Organism |
---|---|
isolation of the cDNAs encoding MnP isoenzymes: coding sequences of IZ-MnP1 cDNA with 1152 bp and IZ-MnP2 cDNA with 1155 bp in length, 2 copies of DNA encoding MnP isoenzymes exist in genomic DNA, 4 isoenzymes are generated as products of 2 genes by the difference of posttranslational modification | Deuteromycotina sp. |
Crystallization (Comment) | Organism |
---|---|
crystal structure | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0447 | - |
Mn2+ | - |
Deuteromycotina sp. | |
0.0537 | - |
Mn2+ | - |
Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
extracellular | - |
Deuteromycotina sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | MnP has 2 structural calcium ions | Phanerodontia chrysosporium | |
Ca2+ | MnP has 2 structural calcium ions | Deuteromycotina sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Deuteromycotina sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | important component of lignin degradation system | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Deuteromycotina sp. | important component of lignin degradation system | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium ME446 | important component of lignin degradation system | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deuteromycotina sp. | - |
fungi IZU-154, FERM BP-1859, hyperlignolytic fungus IZU-154, belongs to the family Deuteromycotina and is deposited as the strain name of NK-1148, 4 isoenzymes | - |
Phanerodontia chrysosporium | - |
- |
- |
Phanerodontia chrysosporium | - |
white rot basidomycete | - |
Phanerodontia chrysosporium ME446 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | Asn-130 follows general rule of the N-glycosylation site | Deuteromycotina sp. |
glycoprotein | with 3 potential N-glycosylation sites, but only Asn-131 binds to carbohydrate | Phanerodontia chrysosporium |
proteolytic modification | leader sequence at the N-terminus | Phanerodontia chrysosporium |
proteolytic modification | leader sequence at the N-terminus: first 24 amino acids possess characteristics of signal peptides | Deuteromycotina sp. |
Purification (Comment) | Organism |
---|---|
- |
Phanerodontia chrysosporium |
- |
Deuteromycotina sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Phanerodontia chrysosporium |
additional information | - |
- |
Deuteromycotina sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | - |
Deuteromycotina sp. | Mn3+ + H2O | product Mn3+ possibly migrates into polymer molecules, such as lignin, nylon and melanin, and initiates nonspecific oxidation | ? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | - |
Deuteromycotina sp. | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Deuteromycotina sp. | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium ME446 | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Phanerodontia chrysosporium ME446 | Mn3+ + H2O | - |
? | |
additional information | structural properties | Phanerodontia chrysosporium | ? | - |
? | |
additional information | structural properties | Deuteromycotina sp. | ? | - |
? | |
additional information | structural properties | Phanerodontia chrysosporium ME446 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Phanerodontia chrysosporium |
? | x * 43000, SDS-PAGE | Deuteromycotina sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Phanerodontia chrysosporium |
30 | - |
assay at | Deuteromycotina sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
125 | - |
Mn2+ | - |
Phanerodontia chrysosporium | |
161 | - |
Mn2+ | - |
Deuteromycotina sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Phanerodontia chrysosporium |
additional information | - |
4 isoenzymes: pI 5.1, 4.9, 4.5 and 3.7 | Deuteromycotina sp. |