Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-hydroxy acid | stimulates by chelating Mn3+ and stabilizing its high redox potential | Phanerodontia chrysosporium | |
citrate | stimulates by chelating Mn3+ and stabilizing its high redox potential | Phanerodontia chrysosporium | |
H2O2 | H2O2-dependent | Phanerodontia chrysosporium | |
L-malate | activates by chelating and stabilizing Mn3+ | Phanerodontia chrysosporium | |
L-Tartrate | - |
Phanerodontia chrysosporium | |
Lactate | stimulates by complexing with and stabilizing Mn3+ | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
Mn2+ | - |
Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | involved in lignin-degradation | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | acts together with lignin peroxidase in lignin-degradation of white rot fungi | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | in absence of H2O2 it may play a role in fungal peroxide production under ligninolytic conditions | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Phanerodontia chrysosporium OGC101 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
12fold purification | Phanerodontia chrysosporium |
3 isozymes | Phanerodontia chrysosporium |
Storage Stability | Organism |
---|---|
-20°C, concentrated preparation, 1 mg/ml, 3 months, stable | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | product Mn3+ is a nonspecific oxidant which in turn oxidizes a variety of organic compounds | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | acts together with lignin peroxidase in lignin-degradation of white rot fungi | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | in absence of H2O2 it may play a role in fungal peroxide production under ligninolytic conditions | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Phanerodontia chrysosporium | ? | - |
? | |
additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phanerodontia chrysosporium | ? | - |
? | |
additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+, generating H2O2 for oxidizing other substrates | Phanerodontia chrysosporium | ? | - |
? | |
additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ | Phanerodontia chrysosporium | ? | - |
? | |
additional information | no other metal can substitute Mn2+ | Phanerodontia chrysosporium | ? | - |
? | |
additional information | in presence of Mn2+ enzyme oxidizes various organic compounds | Phanerodontia chrysosporium | ? | - |
? | |
additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+, generating H2O2 for oxidizing other substrates | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
additional information | no other metal can substitute Mn2+ | Phanerodontia chrysosporium OGC101 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay at room temperature | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Phanerodontia chrysosporium |