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Literature summary for 1.11.1.11 extracted from

  • Hartmann, J.; Asch, F.
    Extraction, storage duration, and storage temperature affect the activity of ascorbate peroxidase, glutathione reductase, and superoxide dismutase in rice tissue (2019), Biology, 8, 70 .
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
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Oryza sativa 5829
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Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in the heme group Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+ Oryza sativa
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L-ascorbate + L-dehydroascorbate + 2 H2O
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?

Organism

Organism UniProt Comment Textmining
Oryza sativa
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var. IR31785 and var. Suakoko8, obtained from AfricaRice, Benin
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Source Tissue

Source Tissue Comment Organism Textmining
leaf blades and sheaths Oryza sativa
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additional information APX activity is highest in the leaf blades and tends to be higher in older than in younger plant parts Oryza sativa
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root
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Oryza sativa
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme activity of ascorbate peroxidase (APX), for 2 varieties, Suakoko8 and IR31785, in samples of the leaf blade, the sheath of the youngest fully developed leaf of the main culm, the blade and sheath of the third youngest fully developed leaf of the main culm, and the root, samples are fresh or stored for 1-12 weeks frozen, or freeze-dried, overview Oryza sativa

Storage Stability

Storage Stability Organism
effect of dfferent storage methods such as freeze-drying, freezing at -20°C, and freezing at -80°C on the activity of enzyme ascorbate peroxidase from two rice varieties, overview. When subjected to different storage methods, there are no differences between varieties, but strong effects of the different storage methods on enzyme activities are found strongly differing between extracts from stored tissue samples or extracts stored from freshly sampled material. Enzyme-specific storage methods and durations allow for expanding the window for analyses in large experimental studies involving destructive samplings for enzyme kinetics Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbate + H2O2 + 2 H+
-
Oryza sativa L-ascorbate + L-dehydroascorbate + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
APX
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Oryza sativa
ascorbate peroxidase
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Oryza sativa

Cofactor

Cofactor Comment Organism Structure
heme
-
Oryza sativa