Protein Variants | Comment | Organism |
---|---|---|
additional information | chloroperoxidase is successfully entrapped in multi polysaccharide magnetic supports, which are developed based on a multi polysaccharide shell. Leakage of the enzyme is observed with single-shell polysaccharide coating. High CPO reusability is measured with a polymeric double-shell magnetic microsupport. The magnetic cores contain iron oxide nanoparticles, the polymer diminishes the interaction between the magnetic cores themselves, can improve the colloidal stability of the support, and prevent any interaction with the environment that would affect both support properties and enzyme stability. Different magnetic micro-supports, based on polydopamine-coated iron oxide nanoparticles with a multi polysaccharide shell, are developed and evaluated. Method overview. CPO is successfully immobilized with an efficiency of entrapment between 92% and 100% in the case of supports with chitosan in the interior or outer shell respectively. A very good chemical stability of the support under reaction conditions is observed in the case of an interior shell of alginate and an outer coating of chitosan, together with an excellent reusability of the immobilized enzyme, that is recycled to catalyze up to 25 consecutive reaction cycles. Chlorination of monochlorodimedone to dichlorodimedone is used as model reaction for chloroperoxidase catalytic assay | Leptoxyphium fumago |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RH + chloride + H2O2 | Leptoxyphium fumago | - |
RCl + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptoxyphium fumago | P04963 | Caldariomyces fumago | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chlorodimedone + chloride + H2O2 | model substrate monochlorodimedone | Leptoxyphium fumago | 1,1-dimethyl-4,4-dichloro-3,5-cyclohexanedione + 2 H2O | - |
? | |
RH + chloride + H2O2 | - |
Leptoxyphium fumago | RCl + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chloroperoxidase | - |
Leptoxyphium fumago |
CPO | - |
Leptoxyphium fumago |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Leptoxyphium fumago |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.9 | - |
assay at | Leptoxyphium fumago |