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Literature summary for 1.11.1.1 extracted from

  • Riebe, O.; Fischer, R.J.; Wampler, D.A.; Kurtz, D.M.; Bahl, H.
    Pathway for H2O2 and O2 detoxification in Clostridium acetobutylicum (2009), Microbiology, 155, 16-24.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the rbr3B gene is cloned into a pThydA vector for expression of the protein in Escherichia coli DH5alpha cells and in Clostridium acetobutylicum ATCC 824 Clostridium acetobutylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
determined by SDS-PAGE Clostridium acetobutylicum
21580
-
theoretical Clostridium acetobutylicum
80000
-
determined by gel filtration Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + H2O2 Clostridium acetobutylicum
-
NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum Q97D83
-
-

Purification (Commentary)

Purification (Comment) Organism
the crude extract is loaded onto columns containing a Strep-Tactin-Sepharose matrix Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.017
-
Clostridium acetobutylicum wild-type cell lysate Clostridium acetobutylicum
0.024
-
Clostridium acetobutylicum pTrbr3B cell lysate Clostridium acetobutylicum
0.66
-
NADH:rubredoxin oxidoreductase, revRbr Clostridium acetobutylicum
0.993
-
NADH:rubredoxin oxidoreductase, rubredoxin, revRbr Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + H2O2
-
Clostridium acetobutylicum NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 21000 Clostridium acetobutylicum

Synonyms

Synonyms Comment Organism
NADH oxidase
-
Clostridium acetobutylicum
NADH peroxidase
-
Clostridium acetobutylicum
NADH:H2O2 oxidoreductase
-
Clostridium acetobutylicum
NADH:O2 oxidoreductase
-
Clostridium acetobutylicum
non-haem diiron protein
-
Clostridium acetobutylicum
reverse rubrerythrin
-
Clostridium acetobutylicum
revRbr
-
Clostridium acetobutylicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
activity assay at room temperature Clostridium acetobutylicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
activity assay Clostridium acetobutylicum

Cofactor

Cofactor Comment Organism Structure
rubredoxin rubredoxin-like Fe(SCys)4-domain Clostridium acetobutylicum

Expression

Organism Comment Expression
Clostridium acetobutylicum revRbr is massively up-regulated in response to oxidative stress up