Cloned (Comment) | Organism |
---|---|
the rbr3B gene is cloned into a pThydA vector for expression of the protein in Escherichia coli DH5alpha cells and in Clostridium acetobutylicum ATCC 824 | Clostridium acetobutylicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
determined by SDS-PAGE | Clostridium acetobutylicum |
21580 | - |
theoretical | Clostridium acetobutylicum |
80000 | - |
determined by gel filtration | Clostridium acetobutylicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + H2O2 | Clostridium acetobutylicum | - |
NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | Q97D83 | - |
- |
Purification (Comment) | Organism |
---|---|
the crude extract is loaded onto columns containing a Strep-Tactin-Sepharose matrix | Clostridium acetobutylicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.017 | - |
Clostridium acetobutylicum wild-type cell lysate | Clostridium acetobutylicum |
0.024 | - |
Clostridium acetobutylicum pTrbr3B cell lysate | Clostridium acetobutylicum |
0.66 | - |
NADH:rubredoxin oxidoreductase, revRbr | Clostridium acetobutylicum |
0.993 | - |
NADH:rubredoxin oxidoreductase, rubredoxin, revRbr | Clostridium acetobutylicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + H2O2 | - |
Clostridium acetobutylicum | NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 21000 | Clostridium acetobutylicum |
Synonyms | Comment | Organism |
---|---|---|
NADH oxidase | - |
Clostridium acetobutylicum |
NADH peroxidase | - |
Clostridium acetobutylicum |
NADH:H2O2 oxidoreductase | - |
Clostridium acetobutylicum |
NADH:O2 oxidoreductase | - |
Clostridium acetobutylicum |
non-haem diiron protein | - |
Clostridium acetobutylicum |
reverse rubrerythrin | - |
Clostridium acetobutylicum |
revRbr | - |
Clostridium acetobutylicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
activity assay at room temperature | Clostridium acetobutylicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
activity assay | Clostridium acetobutylicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
rubredoxin | rubredoxin-like Fe(SCys)4-domain | Clostridium acetobutylicum |
Organism | Comment | Expression |
---|---|---|
Clostridium acetobutylicum | revRbr is massively up-regulated in response to oxidative stress | up |