Application | Comment | Organism |
---|---|---|
synthesis | the enzyme will serve as a useful tool for enzymatic polymerization of diphenolic compounds such as caffeic acid and ferulic acid | Agaricus brasiliensis |
Cloned (Comment) | Organism |
---|---|
attempts of heterologous expression of the wild-type laccase using a Pichia pastoris secretory expression system are unsuccessful most likely because the enzyme is too unstable and degrades immediately after production. Therefore the stability of the laccase is improved by using a phylogeny-based design method. A mutant laccase is created in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence. The resulting mutant protein is successfully produced using the Pichia pastoris secretory expression system and then purified | Agaricus brasiliensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus brasiliensis | V5XVN3 | - |
- |
Purification (Comment) | Organism |
---|---|
attempts of heterologous expression of the wild-type laccase using a Pichia pastoris secretory expression system are unsuccessful most likely because the enzyme is too unstable and degrades immediately after production. Therefore the stability of the laccase is improved by using a phylogeny-based design method. A mutant laccase is created in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence. The resulting mutant protein is successfully produced using the Pichia pastoris secretory expression system and then purified | Agaricus brasiliensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Agaricus brasiliensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lac2a | - |
Agaricus brasiliensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the mutant enzyme is resistant to heat treatment of 10 min. The mutant protein loses 45% of its original activity after incubation for 1 h. Mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence | Agaricus brasiliensis |
80 | - |
10 min, mutant enzyme retains 70% of its original activity, mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence | Agaricus brasiliensis |
85 | - |
10 min, mutant enzyme retains 30% of its original activity, mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence | Agaricus brasiliensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
half live of mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence: 63.0 min (at pH 6.0), 6.1 min (pH 10.5) | Agaricus brasiliensis |
7.6 | - |
half live of mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence: 57.8 min (at pH 6.0) | Agaricus brasiliensis |