Cloned (Comment) | Organism |
---|---|
gene CcLCC5I, expresssion in Pichia pastoris GS115 | Coprinopsis cinerea |
General Stability | Organism |
---|---|
the activity of CcLCC5I is stable after incubation for 16 h at 4°C in 0.2 M citrate-phosphate buffer at weakly alkaline conditions, the minimal effect on enzyme activity is at pH 8.6 | Coprinopsis cinerea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
Ba2+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
Ca2+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
Cr3+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
dithiothreitol | - |
Coprinopsis cinerea | |
Fe2+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
Fe3+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
K+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
L-cysteine | - |
Coprinopsis cinerea | |
Li+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
Mg2+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
Mn2+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
additional information | except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity | Coprinopsis cinerea | |
Na+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea | |
NaN3 | - |
Coprinopsis cinerea | |
sodium dodecylsulfate | - |
Coprinopsis cinerea | |
sodium sulfite | - |
Coprinopsis cinerea | |
sodium thiosulfate | - |
Coprinopsis cinerea | |
Zn2+ | activating at 1 mM, inhibitory at 10 mM | Coprinopsis cinerea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.086 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 30°C | Coprinopsis cinerea | |
17.6 | - |
guaiacol | pH 3.0, 30°C | Coprinopsis cinerea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity | Coprinopsis cinerea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56800 | - |
x * 56800, SDS-PAGE | Coprinopsis cinerea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | A8N3P5 | gene CcLCC5I | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Coprinopsis cinerea | ? | - |
? | |
guaiacol + O2 | - |
Coprinopsis cinerea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56800, SDS-PAGE | Coprinopsis cinerea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Coprinopsis cinerea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | optimum temperature of CcLCC5I is 55°C, the activity of laccase is high from 30 to 50°C, when the temperature is over 55°C, the activity declines more, when the temperature reaches 70°C, the relative activity of Lcc is only 23%, profile, overview | Coprinopsis cinerea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme, stable up to | Coprinopsis cinerea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.211 | - |
guaiacol | pH 3.0, 30°C | Coprinopsis cinerea | |
4.22 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 30°C | Coprinopsis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Coprinopsis cinerea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 7 | activity range, profile, overview | Coprinopsis cinerea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
guaiacol | pH 3.0, 30°C | Coprinopsis cinerea | |
49.1 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 30°C | Coprinopsis cinerea |