Application | Comment | Organism |
---|---|---|
biofuel production | application in enzymatic fuel cells is limited by their relatively low activity with this substrate | Humicola insolens |
biofuel production | application in enzymatic fuel cells is limited by their relatively low activity with this substrate | Thermothelomyces fergusii |
Cloned (Comment) | Organism |
---|---|
expression in Aspergillus oryzae | Humicola insolens |
expression in Pichia pastoris X-33 cells | Thermothelomyces fergusii |
Protein Variants | Comment | Organism |
---|---|---|
C285Y | mutation causes a 5.6time decrease in KM for glucose and a decreased affinity for the other disaccharides. Overall catalytic efficiency increases 6.7times as compared to the wild type enzyme | Humicola insolens |
C291Y | mutation causes a 8.3times decrease in KM for glucose and a decreased affinity for the other disaccharides | Thermothelomyces fergusii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
cellobiose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
0.0078 | - |
cellobiose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
0.0205 | - |
lactose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
0.036 | - |
lactose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
0.06 | - |
cellobiose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
0.28 | - |
cellobiose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
0.44 | - |
lactose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
1.14 | - |
lactose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
2 | - |
maltose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
2.7 | - |
maltose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
5.2 | - |
D-glucose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
9.3 | - |
D-glucose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
22.8 | - |
maltose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
27.8 | - |
D-glucose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
81 | - |
maltose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
88.2 | - |
D-glucose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Humicola insolens | - |
- |
extracellular | - |
Thermothelomyces fergusii | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humicola insolens | Q9P8H5 | - |
- |
Thermothelomyces fergusii | E7D6B9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Humicola insolens |
- |
Thermothelomyces fergusii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + cytochrome c | - |
Humicola insolens | cellobiono-1,5-lactone + reduced cytochrome c | - |
? | |
cellobiose + cytochrome c | - |
Thermothelomyces fergusii | cellobiono-1,5-lactone + reduced cytochrome c | - |
? | |
D-glucose + cytochrome c | - |
Humicola insolens | D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
D-glucose + cytochrome c | - |
Thermothelomyces fergusii | D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
lactose + cytochrome c | - |
Humicola insolens | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
lactose + cytochrome c | - |
Thermothelomyces fergusii | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
maltose + cytochrome c | - |
Humicola insolens | 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
maltose + cytochrome c | - |
Thermothelomyces fergusii | 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Humicola insolens |
Cdh | - |
Thermothelomyces fergusii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
maltose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
0.14 | - |
maltose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
0.23 | - |
D-glucose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
0.26 | - |
cellobiose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
0.29 | - |
D-glucose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
0.33 | - |
lactose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
0.4 | - |
maltose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
0.4 | - |
maltose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
0.44 | - |
cellobiose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
0.5 | - |
lactose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
2 | - |
cellobiose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
2.2 | - |
D-glucose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
2.3 | - |
lactose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
2.36 | - |
lactose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
2.44 | - |
cellobiose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
3.3 | - |
D-glucose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Humicola insolens |
7.4 | - |
- |
Thermothelomyces fergusii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
maltose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
0.0083 | - |
D-glucose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
0.0175 | - |
maltose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
0.025 | - |
D-glucose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
0.0265 | - |
maltose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
0.0558 | - |
D-glucose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
0.148 | - |
maltose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
0.355 | - |
D-glucose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
0.44 | - |
lactose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
1.6 | - |
cellobiose | pH 7.5, 25°C, mutant enzyme C285Y | Humicola insolens | |
5.4 | - |
lactose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
16.1 | - |
lactose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
40.6 | - |
cellobiose | pH 7.5, 25°C, mutant enzyme C291Y | Thermothelomyces fergusii | |
63.8 | - |
lactose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii | |
68.4 | - |
cellobiose | pH 7.5, 25°C, wild-type enzyme | Humicola insolens | |
256 | - |
cellobiose | pH 7.5, 25°C, wild-type enzyme | Thermothelomyces fergusii |