Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus niger | Coprinopsis cinerea |
expressed in Aspergillus niger | Podospora anserina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
cellotriose | at 6.0 pH and 30 °C | Podospora anserina | |
0.041 | - |
cellotetraose | at 6.0 pH and 30 °C | Podospora anserina | |
0.043 | - |
lactose | at 6.0 pH and 30 °C | Podospora anserina | |
0.132 | - |
cellobiose | at 6.0 pH and 30 °C | Podospora anserina | |
0.156 | - |
cellobiose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
0.281 | - |
cellotetraose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
0.352 | - |
cellotriose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
1.523 | - |
lactose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
328.6 | - |
D-glucose | at 6.0 pH and 30 °C | Podospora anserina | |
354.4 | - |
D-glucose | at 5.0 pH and 30 °C | Coprinopsis cinerea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
x * 83000, SDS-PAGE | Coprinopsis cinerea |
83000 | - |
x * 83000, SDS-PAGE | Podospora anserina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | - |
- |
- |
Podospora anserina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chelating Sepharose column chromatography | Coprinopsis cinerea |
chelating Sepharose column chromatography | Podospora anserina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Coprinopsis cinerea | - |
mycelium | - |
Podospora anserina | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenolindophenol | - |
Coprinopsis cinerea | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellobiose + 2,6-dichlorophenolindophenol | - |
Podospora anserina | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellotetraose + 2,6-dichlorophenolindophenol | - |
Coprinopsis cinerea | cellotetraono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellotetraose + 2,6-dichlorophenolindophenol | - |
Podospora anserina | cellotetraono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellotriose + 2,6-dichlorophenolindophenol | - |
Coprinopsis cinerea | cellotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellotriose + 2,6-dichlorophenolindophenol | - |
Podospora anserina | cellotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Coprinopsis cinerea | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Podospora anserina | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + 2,6-dichlorophenolindophenol | - |
Coprinopsis cinerea | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + 2,6-dichlorophenolindophenol | - |
Podospora anserina | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 83000, SDS-PAGE | Coprinopsis cinerea |
? | x * 83000, SDS-PAGE | Podospora anserina |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Coprinopsis cinerea |
Cdh | - |
Podospora anserina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Coprinopsis cinerea |
65 | 70 | - |
Podospora anserina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme is thermostable at 30°C and loses approximately 20-25% of its activity after 4 h at 40°C and half of its activity after 17 min at 50°C | Podospora anserina |
30 | 50 | the enzyme is thermostable at 30°C and loses approximately 20-25% of its activity after 4 h at 40°C and half of its activity after 30 min at 50°C | Coprinopsis cinerea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
D-glucose | at 6.0 pH and 30 °C | Podospora anserina | |
2.6 | - |
lactose | at 6.0 pH and 30 °C | Podospora anserina | |
3.8 | - |
cellotriose | at 6.0 pH and 30 °C | Podospora anserina | |
4 | - |
cellotetraose | at 6.0 pH and 30 °C | Podospora anserina | |
8.56 | - |
cellobiose | at 6.0 pH and 30 °C | Podospora anserina | |
34.4 | - |
D-glucose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
96.9 | - |
cellobiose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
101.6 | - |
cellotetraose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
136.3 | - |
lactose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
156 | - |
cellotriose | at 5.0 pH and 30 °C | Coprinopsis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Coprinopsis cinerea |
6 | - |
- |
Podospora anserina |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
the enzyme retains its activity after 2 and 24 h at pH 7.0 | Coprinopsis cinerea |
7 | - |
the enzyme retains its activity after 2 and 24 h at pH 7.0 | Podospora anserina |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00494 | - |
D-glucose | at 6.0 pH and 30 °C | Podospora anserina | |
0.097 | - |
D-glucose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
60 | - |
lactose | at 6.0 pH and 30 °C | Podospora anserina | |
64 | - |
cellobiose | at 6.0 pH and 30 °C | Podospora anserina | |
89.4 | - |
lactose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
99 | - |
cellotetraose | at 6.0 pH and 30 °C | Podospora anserina | |
142 | - |
cellotriose | at 6.0 pH and 30 °C | Podospora anserina | |
361 | - |
cellotetraose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
442 | - |
cellotriose | at 5.0 pH and 30 °C | Coprinopsis cinerea | |
621 | - |
cellobiose | at 5.0 pH and 30 °C | Coprinopsis cinerea |