Cloned (Comment) | Organism |
---|---|
expressed in Neurospora crassa strain 2489 | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00579 | - |
cellobiose | isoform CDH2, at pH 6.0 and 50°C | Neurospora crassa | |
0.02572 | - |
cellobiose | isoform CDH1, at pH 6.0 and 50°C | Neurospora crassa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Neurospora crassa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
1 * 86000, mature peptides, calculated from amino acid sequence | Neurospora crassa |
95000 | - |
1 * 95000, deglycosylated enzyme forms, SDS-PAGE | Neurospora crassa |
100000 | - |
1 * 100000, isoform CDH1, SDS-PAGE | Neurospora crassa |
130000 | - |
1 * 130000, isoform CDH2, SDS-PAGE | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Neurospora crassa FGSC 2489 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Neurospora crassa |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Sephacryl S-200 gel filtration | Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:avicel-grown cell | - |
Neurospora crassa | - |
culture condition:rice straw-grown cell | - |
Neurospora crassa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
isoform CDH2 from extracellular medium, at pH 6.0 and 50°C | Neurospora crassa |
1.19 | - |
isoform CDH1 from extracellular medium, at pH 6.0 and 50°C | Neurospora crassa |
7.93 | - |
isoform CDH2 after 28.32fold purification, at pH 6.0 and 50°C | Neurospora crassa |
8.89 | - |
isoform CDH1 after 7.47fold purification, at pH 6.0 and 50°C | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenol indophenol | - |
Neurospora crassa | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
cellobiose + 2,6-dichlorophenol indophenol | - |
Neurospora crassa FGSC 2489 | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
additional information | isoform CDH2 lacks the cellulose-binding module and contributes marginally to total enzyme activity but has specific activity similar to that of isoform CDH1 and a much lower Km value | Neurospora crassa | ? | - |
? | |
additional information | isoform CDH2 lacks the cellulose-binding module and contributes marginally to total enzyme activity but has specific activity similar to that of isoform CDH1 and a much lower Km value | Neurospora crassa FGSC 2489 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, isoform CDH1, SDS-PAGE | Neurospora crassa |
monomer | 1 * 130000, isoform CDH2, SDS-PAGE | Neurospora crassa |
monomer | 1 * 86000, mature peptides, calculated from amino acid sequence | Neurospora crassa |
monomer | 1 * 95000, deglycosylated enzyme forms, SDS-PAGE | Neurospora crassa |
Synonyms | Comment | Organism |
---|---|---|
CDH1 | isoform | Neurospora crassa |
Cdh2 | isoform | Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Neurospora crassa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | about 70% activity between 45 and 55°C | Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | the enzymes are stable for 10 h at 20-30°C | Neurospora crassa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.7 | - |
cellobiose | isoform CDH2, at pH 6.0 and 50°C | Neurospora crassa | |
13.5 | - |
cellobiose | isoform CDH1, at pH 6.0 and 50°C | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | about 50% activity between pH 5.0 and 7.5 | Neurospora crassa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | enzyme activities decrease sharply when stored in buffers with pHs below 3.0 or above 8.0 | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Neurospora crassa | |
heme | - |
Neurospora crassa |
Organism | Comment | Expression |
---|---|---|
Neurospora crassa | the enzyme is produced when avicel or rice straw is used as the carbon source | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
525 | - |
cellobiose | isoform CDH1, at pH 6.0 and 50°C | Neurospora crassa | |
2193 | - |
cellobiose | isoform CDH2, at pH 6.0 and 50°C | Neurospora crassa |