Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
L-2-hydroxyglutarate | at pH 7.0 and 25°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-hydroxyglutarate + O2 | Escherichia coli | - |
2-oxoglutarate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-Sepharose 6 column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-hydroxyglutarate + O2 | - |
Escherichia coli | 2-oxoglutarate + H2O2 | - |
? | |
additional information | no activity with dimethylglycine, sarcosine, butyraldehyde or 3-hydroxybutyric acid. Most 2-hydroxy acids are ineffective as substrates, including L-malic acid, DL-malic acid, DL-lactic acid, L-mandelic acid, D-mandelic acid, and 2-hydroxycaproic acid | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47640, His-tagged protein, calculated from amino acid sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
L-2-hydroxyglutarate oxygenase | - |
Escherichia coli |
lhgO | - |
Escherichia coli |
YgaF | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
L-2-hydroxyglutarate | at pH 7.0 and 25°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains a noncovalently bound FAD | Escherichia coli | |
additional information | not affected by NAD+ or NADP+ | Escherichia coli |