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Literature summary for 1.1.3.21 extracted from
- Functional and antigenic properties of GlpO from Mycoplasma mycoides subsp. mycoides SC: Characterization of a flavin adenine dinucleotide-binding site deletion mutant (2009), Vet. Res., 40, 35.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycoplasma mycoides subsp. mycoides |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | recombinant GlpO lacking the six amino acids Gly12-Gly13-Gly14-Ile15-Ile16-Gly17 (GlpODELTAFAD) is unable to bind FAD and is also devoid of glycerophosphate oxidase activity | Mycoplasma mycoides subsp. mycoides |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
two bands at 45000 and 90000 Da, SDS-PAGE | Mycoplasma mycoides subsp. mycoides |
| 90000 | - |
two bands at 45000 and 90000 Da, SDS-PAGE | Mycoplasma mycoides subsp. mycoides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycoplasma mycoides subsp. mycoides | Q70GP9 | - |
- |
| Mycoplasma mycoides subsp. mycoides SC Afade | Q70GP9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Mycoplasma mycoides subsp. mycoides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol 3-phosphate + O2 | - |
Mycoplasma mycoides subsp. mycoides | dihydroxyacetone phosphate + H2O2 | - |
? |  |
| glycerol 3-phosphate + O2 | - |
Mycoplasma mycoides subsp. mycoides SC Afade | dihydroxyacetone phosphate + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-glycerol-3-phosphate oxidase | - |
Mycoplasma mycoides subsp. mycoides |
| GlpO | - |
Mycoplasma mycoides subsp. mycoides |
| L-alpha-glycerophosphate oxidase | - |
Mycoplasma mycoides subsp. mycoides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Mycoplasma mycoides subsp. mycoides | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | GlpO is involved in production and translocation of toxic H2O2 into the host cell, causing inflammation and cell death | Mycoplasma mycoides subsp. mycoides |
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Release 2023.1 (January 2023)
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