Literature summary for 1.1.3.21 extracted from

Mackova, M.; Kostal, J.; Demnerova, K.
Properties and stability of glycerophosphate oxidase isolated from a mutant strain of Aerococcus viridans (2000), Lett. Appl. Microbiol., 30, 188-191.

Activating Compound

Activating Compound	Comment	Organism	Structure
glycerol	strain DBM 1509, 50%	Aerococcus viridans
Triton X-100	strain DBM 1509, 1%	Aerococcus viridans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sn-glycerol-3-phosphate + O2	Aerococcus viridans	-	glycerone phosphate + H2O2	i.e. dihydroxyacetone phosphate	?
sn-glycerol-3-phosphate + O2	Aerococcus viridans DBM 1509	-	glycerone phosphate + H2O2	i.e. dihydroxyacetone phosphate	?

Organism

Organism	UniProt	Comment	Textmining
Aerococcus viridans	-	-	-
Aerococcus viridans DBM 1509	-	-	-

Storage Stability

Storage Stability	Organism
-20°C, 40 d, 13% loss of activity, 4°C, 14 d, 92% loss of activity, 30°, 24 h, 90% loss of activity, 40°C, 80 min, 80% loss of activity, 4°C, 0.2% dithiothreitol, 20 d, 32% loss of activity, 4°C, 0.2% mercaptoethanol, 20 d, 34% loss of activity, 4°C, 50% glycerol, 20 d, 36% loss of activity, 4°C, 100 mM EDTA, 20 d, 24% loss of activity, 4°C, 2.5% polyethylenglycol, 20 d, 63% loss of activity, 4°C, lyophilization, 2% dextrin II 14 d, 35% loss of activity, 20°C, lyophilization, 2% dextrin II, 14 d, 60% loss of activity, 37°C, lyophilization, 2% dextrin II, 14 d, 85% loss of activity	Aerococcus viridans

Organism

-20°C, 40 d, 13% loss of activity, 4°C, 14 d, 92% loss of activity, 30°, 24 h, 90% loss of activity, 40°C, 80 min, 80% loss of activity, 4°C, 0.2% dithiothreitol, 20 d, 32% loss of activity, 4°C, 0.2% mercaptoethanol, 20 d, 34% loss of activity, 4°C, 50% glycerol, 20 d, 36% loss of activity, 4°C, 100 mM EDTA, 20 d, 24% loss of activity, 4°C, 2.5% polyethylenglycol, 20 d, 63% loss of activity, 4°C, lyophilization, 2% dextrin II 14 d, 35% loss of activity, 20°C, lyophilization, 2% dextrin II, 14 d, 60% loss of activity, 37°C, lyophilization, 2% dextrin II, 14 d, 85% loss of activity

Aerococcus viridans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sn-glycerol-3-phosphate + O2	-	Aerococcus viridans	glycerone phosphate + H2O2	i.e. dihydroxyacetone phosphate	?
sn-glycerol-3-phosphate + O2	-	Aerococcus viridans DBM 1509	glycerone phosphate + H2O2	i.e. dihydroxyacetone phosphate	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	strain DBM 1509	Aerococcus viridans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	strain DBM 1509	Aerococcus viridans

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
9	-	strain DBM 1509	Aerococcus viridans

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Aerococcus viridans