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Literature summary for 1.1.3.21 extracted from

  • Mackova, M.; Kostal, J.; Demnerova, K.
    Properties and stability of glycerophosphate oxidase isolated from a mutant strain of Aerococcus viridans (2000), Lett. Appl. Microbiol., 30, 188-191.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glycerol strain DBM 1509, 50% Aerococcus viridans
Triton X-100 strain DBM 1509, 1% Aerococcus viridans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol-3-phosphate + O2 Aerococcus viridans
-
glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 Aerococcus viridans DBM 1509
-
glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Organism

Organism UniProt Comment Textmining
Aerococcus viridans
-
-
-
Aerococcus viridans DBM 1509
-
-
-

Storage Stability

Storage Stability Organism
-20°C, 40 d, 13% loss of activity, 4°C, 14 d, 92% loss of activity, 30°, 24 h, 90% loss of activity, 40°C, 80 min, 80% loss of activity, 4°C, 0.2% dithiothreitol, 20 d, 32% loss of activity, 4°C, 0.2% mercaptoethanol, 20 d, 34% loss of activity, 4°C, 50% glycerol, 20 d, 36% loss of activity, 4°C, 100 mM EDTA, 20 d, 24% loss of activity, 4°C, 2.5% polyethylenglycol, 20 d, 63% loss of activity, 4°C, lyophilization, 2% dextrin II 14 d, 35% loss of activity, 20°C, lyophilization, 2% dextrin II, 14 d, 60% loss of activity, 37°C, lyophilization, 2% dextrin II, 14 d, 85% loss of activity Aerococcus viridans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol-3-phosphate + O2
-
Aerococcus viridans glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2
-
Aerococcus viridans DBM 1509 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
strain DBM 1509 Aerococcus viridans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
strain DBM 1509 Aerococcus viridans

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
strain DBM 1509 Aerococcus viridans

Cofactor

Cofactor Comment Organism Structure
FAD
-
Aerococcus viridans