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Literature summary for 1.1.3.21 extracted from
- Studies on the structure and mechanism of Streptococcus faecium L-alpha-glycerophosphate oxidase (1986), J. Biol. Chem., 261, 14398-14407.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
2 * 65000, SDS-PAGE | Enterococcus faecium |
| 123000 | - |
strain ATCC 12755, sedimentation equilibrium | Enterococcus faecium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sn-glycerol-3-phosphate + O2 | Enterococcus faecium | - |
glycerone phosphate + H2O2 | i.e. dihydroxyacetone phosphate | ? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecium | - |
ATCC 12755 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterococcus faecium |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| sn-glycerol 3-phosphate + O2 = glycerone phosphate + H2O2 | mechanism | Enterococcus faecium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62 | - |
strain ATCC 12755 | Enterococcus faecium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sn-glycerol 3-phosphate + O2 | - |
Enterococcus faecium | glycerone phosphate + H2O2 | - |
r | |
| sn-glycerol-3-phosphate + O2 | - |
Enterococcus faecium | glycerone phosphate + H2O2 | i.e. dihydroxyacetone phosphate | ? | |
| sn-glycerol-3-phosphate + O2 | other electron acceptors: ferricyanide | Enterococcus faecium | glycerone phosphate + H2O2 | i.e. dihydroxyacetone phosphate | ? | |
| sn-glycerol-3-phosphate + O2 | ferricyanide, in absence of O2 | Enterococcus faecium | glycerone phosphate + H2O2 | i.e. dihydroxyacetone phosphate | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 65000, SDS-PAGE | Enterococcus faecium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 23.8 | - |
sn-glycerol 3-phosphate | strain ATCC 12755 | Enterococcus faecium | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | prosthetic group | Enterococcus faecium | |
| FAD | 2 mol bound per mol of enzyme | Enterococcus faecium | |
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