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Literature summary for 1.1.3.21 extracted from
- Purification and properties of L-alpha-glycerophosphate oxidase from Streptococcus faecium ATCC 12755 (1979), J. Biol. Chem., 254, 2710-2715.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | increasing the buffer concentration from 10 mM to 100 mM leads to an 2.6-10fold increase in activity, this effect is observed with potassium phosphate, sodium acetate, sodium maleate or other anionic buffers | Enterococcus faecium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fructose-1-phosphate | - |
Enterococcus faecium |  |
| fructose-6-phosphate | - |
Enterococcus faecium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
sn-glycerol-3-phosphate | - |
Enterococcus faecium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 10 mM, 7.7fold activation | Enterococcus faecium | |
| CoCl2 | 10 mM, 10.2fold activation | Enterococcus faecium | |
| KCl | 100 mM, 5.8fold activation | Enterococcus faecium | |
| MgCl2 | 10 mM, 6.8fold activation | Enterococcus faecium | |
| MnCl2 | 10 mM, 7.6 fold activation | Enterococcus faecium | |
| NaCl | 100 mM, 3.8fold activation | Enterococcus faecium | |
| ZnCl2 | 1 mM, 6.3fold activation | Enterococcus faecium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
2 * 72000, SDS-PAGE | Enterococcus faecium |
| 131000 | - |
strain ATCC 12755, gel filtration, sucrose density centrifugation | Enterococcus faecium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sn-glycerol-3-phosphate + O2 | Enterococcus faecium | - |
glycerone phosphate + H2O2 | i.e. dihydroxyacetone phosphate | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecium | - |
ATCC 12755 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterococcus faecium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 85 | 95 | strain ATCC 12755 | Enterococcus faecium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sn-glycerol-3-phosphate + O2 | - |
Enterococcus faecium | glycerone phosphate + H2O2 | i.e. dihydroxyacetone phosphate | ? | |
| sn-glycerol-3-phosphate + O2 | high specificity for L-alpha-glycerophosphate | Enterococcus faecium | glycerone phosphate + H2O2 | i.e. dihydroxyacetone phosphate | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 72000, SDS-PAGE | Enterococcus faecium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Enterococcus faecium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | prosthetic group | Enterococcus faecium | |
| FAD | 2 mol bound per mol of enzyme | Enterococcus faecium | |
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