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Literature summary for 1.1.1.B47 extracted from

  • Kockelkorn, D.; Fuchs, G.
    Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: Enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales (2009), J. Bacteriol., 191, 6352-6362.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta 2(DE3) Metallosphaera sedula

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde. Incubation with 10 mM EDTA for 16 h or with 100 mM EDTA for 1 h at temperatures up to 65°C does not inactivate the enzyme, nor does addition of 10 mM EDTA to the assay mixture inhibit enzyme activity Metallosphaera sedula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
NADPH 65°C, pH 7.9 Metallosphaera sedula
0.052
-
succinate semialdehyde 65°C, pH 7.9, cosubstrate: NADPH Metallosphaera sedula
0.18
-
NADH 65°C, pH 7.9 Metallosphaera sedula

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no stimulation by Mg2+ or Mn2+ (below 5 mM), or Fe2+ (below 1 mM) Metallosphaera sedula
Zn2+ the enzyme contains 1.9 mol of zinc per mol of enzyme monomer Metallosphaera sedula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Metallosphaera sedula
74000
-
gel filtration Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinate semialdehyde + NADPH + H+ Metallosphaera sedula the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea 4-hydroxybutanoate + NADP+
-
?
succinate semialdehyde + NADPH + H+ Metallosphaera sedula DSM 5348 the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea 4-hydroxybutanoate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YGN0
-
-
Metallosphaera sedula DSM 5348 A4YGN0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Metallosphaera sedula

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:autotrophically-grown cell no activity in heterotrophically-grown cell Metallosphaera sedula
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
cell extract, NADH-dependent reaction, 65°C, pH 7.9 Metallosphaera sedula
1.4
-
cell extract, NADPH-dependent reaction, 65°C, pH 7.9 Metallosphaera sedula
700
-
purified enzyme, NADPH-dependent reaction, 65°C, pH 7.9 Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate Metallosphaera sedula ?
-
?
additional information malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate Metallosphaera sedula DSM 5348 ?
-
?
succinate semialdehyde + NADH + H+ very low activity with NADH Metallosphaera sedula 4-hydroxybutanoate + NAD+
-
?
succinate semialdehyde + NADH + H+ very low activity with NADH Metallosphaera sedula DSM 5348 4-hydroxybutanoate + NAD+
-
?
succinate semialdehyde + NADPH + H+
-
Metallosphaera sedula 4-hydroxybutanoate + NADP+
-
?
succinate semialdehyde + NADPH + H+ the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula 4-hydroxybutanoate + NADP+
-
?
succinate semialdehyde + NADPH + H+
-
Metallosphaera sedula DSM 5348 4-hydroxybutanoate + NADP+
-
?
succinate semialdehyde + NADPH + H+ the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula DSM 5348 4-hydroxybutanoate + NADP+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 40000, SDS-PAGE Metallosphaera sedula

Synonyms

Synonyms Comment Organism
Msed_1424 locus name Metallosphaera sedula
succinate semialdehyde reductase
-
Metallosphaera sedula
succinic semialdehyde reductase
-
Metallosphaera sedula
succinic semialdehyde reductase (NADPH)
-
Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Metallosphaera sedula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
440
-
NADPH 65°C, pH 7.9 Metallosphaera sedula
440
-
succinate semialdehyde 65°C, pH 7.9 Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
at 20°C Metallosphaera sedula
7.5
-
at 65°C Metallosphaera sedula

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 at 65°C, half-maximal activity at pH 6.5 and at pH 8.0 Metallosphaera sedula
6.9 8.4 at 20°C, half-maximal activity at pH 6.9 and at pH 8.4 Metallosphaera sedula

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Metallosphaera sedula

General Information

General Information Comment Organism
metabolism the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula