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Literature summary for 1.1.1.B38 extracted from

  • Miller, B.R.; Kung, Y.
    Structural features and domain movements controlling substrate binding and cofactor specificity in class II HMG-CoA reductase (2018), Biochemistry, 57, 654-662 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.B38

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme bound to 3-hydroxy-3-methylglutaryl-CoA, hanging drop vapor diffusion method, using 100 mM Tris pH 8.5, 100-250 mM lithium sulfate, and 15-25% (w/v) PEG 4000. Enzyme bound to NADPH, hanging drop vapor diffusion method, using 100 mM Tris pH 8.5, 100-250 mM lithium sulfate, and 30-40% (w/v) PEG 4000 Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0289
-
 NADPH at pH 7.4 and 37°C Streptococcus pneumoniae
0.153
-
 NADH at pH 7.4 and 37°C Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ Streptococcus pneumoniae
-
 mevalonate + CoA + 2 NAD+
-
 ?
3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Streptococcus pneumoniae
-
 mevalonate + CoA + 2 NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 Streptococcus pneumoniae mevalonate + CoA + 2 NAD+
-
 ?
3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Streptococcus pneumoniae mevalonate + CoA + 2 NADP+
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl coenzyme A reductase
-
 Streptococcus pneumoniae
class II HMG-CoA reductase
-
 Streptococcus pneumoniae
HGMR
-
 Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.131
-
 NADH at pH 7.4 and 37°C Streptococcus pneumoniae
6.85
-
 NADPH at pH 7.4 and 37°C Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Streptococcus pneumoniae
NADPH kinetically preferred cofactor Streptococcus pneumoniae

General Information

General Information Comment Organism
metabolism key enzyme in the mevalonate pathway Streptococcus pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.86
-
 NADH at pH 7.4 and 37°C Streptococcus pneumoniae
240
-
 NADPH at pH 7.4 and 37°C Streptococcus pneumoniae