Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2 mM, 115% of initial activity | Candida viswanathii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxy mercuribenzoate | 2 mM, 5% residual activity | Candida viswanathii | |
AgNO3 | 2 mM, complete inhibition | Candida viswanathii | |
citrate | 2 mM, 28% residual activity | Candida viswanathii | |
Co(NO3)2 | 2 mM, 59% residual activity | Candida viswanathii | |
CuSO4 | 2 mM, complete inhibition | Candida viswanathii | |
D-cysteine | 2 mM, 8% residual activity | Candida viswanathii | |
diethyldicarbonate | 2 mM, 30% residual activity | Candida viswanathii | |
EDTA | 2 mM, 49% residual activity | Candida viswanathii | |
FeCl3 | 2 mM, 2% residual activity | Candida viswanathii | |
glutathione | 2 mM, 31% residual activity | Candida viswanathii | |
guanidinium hydrochloride | 1 M, 1.5% residual activity | Candida viswanathii | |
hydroxylamine | 2 mM, 23% residual activity | Candida viswanathii | |
Hydroxyquinoline | 2 mM, 18% residual activity | Candida viswanathii | |
L-cysteine | 2 mM, 12% residual activity | Candida viswanathii | |
MgCl2 | 2 mM, 75% residual activity | Candida viswanathii | |
MnSO4 | 2 mM, 53% residual activity | Candida viswanathii | |
additional information | not inhibitory at 2 mM: BaCl2, CaCO3, NaCl | Candida viswanathii | |
N-ethylmaleimide | 2 mM, 5% residual activity | Candida viswanathii | |
NiCl2 | 2 mM, 24% residual activity | Candida viswanathii | |
o-phenanthroline | 2 mM, 9% residual activity | Candida viswanathii | |
oxalate | 2 mM, 26% residual activity | Candida viswanathii | |
Pb(OAc)2 | 2 mM, 8% residual activity | Candida viswanathii | |
potassium iodide | 1 M, 1.4% residual activity | Candida viswanathii | |
Sodium azide | 2 mM, 35% residual activity | Candida viswanathii | |
sodium dodecylsulfate | 1% w/v, 6% residual activity | Candida viswanathii | |
tartarate | 2 mM, 21% residual activity | Candida viswanathii | |
Triton X-100 | 1% w/v, 67% residual activity | Candida viswanathii | |
Tween 80 | 1% w/v, 26.5% residual activity | Candida viswanathii | |
Urea | 1 M, 5.8% residual activity | Candida viswanathii | |
ZnSO4 | 2 mM, 2% residual activity | Candida viswanathii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.153 | - |
acetophenone | pH 7.0, 25°C | Candida viswanathii | |
0.157 | - |
NADH | pH 7.0, 25°C | Candida viswanathii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
4 * 29000, SDS-PAGE | Candida viswanathii |
120000 | - |
gel filtration | Candida viswanathii |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl formamide | 15% v/v, 16% residual activity | Candida viswanathii |
dimethyl sulfoxide | 15% v/v, 16% residual activity | Candida viswanathii |
dioxane | 34% v/V, 16% residual activity | Candida viswanathii |
Ethanol | 15% v/v, 48% residual activity | Candida viswanathii |
isopropanol | 15% v/v, 2.5% residual activity | Candida viswanathii |
Pyridine | 15% v/v, 0.4% residual activity | Candida viswanathii |
tetrahydrofuran | 15% v/v, 11% residual activity | Candida viswanathii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida viswanathii | - |
- |
- |
Candida viswanathii MTCC 5158 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida viswanathii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
358.6 | - |
pH 7.0, 25°C | Candida viswanathii |
Storage Stability | Organism |
---|---|
-20°C, phosphate buffer, pH 7.0, presence of 2-mercaptoethanol and phenylmethyl sulfonylfluoride, stable for at least 3 months | Candida viswanathii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-fluoroacetophenone + NADH + H+ | 102% of the activity with acetophenone | Candida viswanathii | (1S)-1-(2-fluorophenyl)ethanol + NAD+ | - |
? | |
2'-fluoroacetophenone + NADH + H+ | 102% of the activity with acetophenone | Candida viswanathii MTCC 5158 | (1S)-1-(2-fluorophenyl)ethanol + NAD+ | - |
? | |
3'-fluoroacetophenone + NADH + H+ | 102% of the activity with acetophenone | Candida viswanathii | (1S)-1-(3-fluorophenyl)ethanol + NAD+ | - |
? | |
3'-fluoroacetophenone + NADH + H+ | 102% of the activity with acetophenone | Candida viswanathii MTCC 5158 | (1S)-1-(3-fluorophenyl)ethanol + NAD+ | - |
? | |
4'-acetoxy acetophenone + NADH + H+ | 80% of the activity with acetophenone | Candida viswanathii | (1S)-1-(4-acetoxyphenyl)ethanol + NAD+ | - |
? | |
4'-acetoxy acetophenone + NADH + H+ | 80% of the activity with acetophenone | Candida viswanathii MTCC 5158 | (1S)-1-(4-acetoxyphenyl)ethanol + NAD+ | - |
? | |
4'-acetyl pyridine + NADH + H+ | 98% of the activity with acetophenone | Candida viswanathii | (1S)-1-(pyridin-4-yl)ethanol + NAD+ | 102% of the activity with acetophenone, enantiomeric excess of more than 99% for (S)-configuration of product | ? | |
4'-acetyl pyridine + NADH + H+ | 98% of the activity with acetophenone | Candida viswanathii MTCC 5158 | (1S)-1-(pyridin-4-yl)ethanol + NAD+ | 102% of the activity with acetophenone, enantiomeric excess of more than 99% for (S)-configuration of product | ? | |
4'-bromoacetophenone + NADH + H+ | 82% of the activity with acetophenone | Candida viswanathii | (1S)-1-(4-bromophenyl)ethanol + NAD+ | - |
? | |
4'-bromoacetophenone + NADH + H+ | 82% of the activity with acetophenone | Candida viswanathii MTCC 5158 | (1S)-1-(4-bromophenyl)ethanol + NAD+ | - |
? | |
4'-chloroacetophenone + NADH + H+ | 83% of the activity with acetophenone | Candida viswanathii | (1S)-1-(4-chlorophenyl)ethanol + NAD+ | - |
? | |
4'-fluoroacetophenone + NADH + H+ | 117% of the activity with acetophenone | Candida viswanathii | (1S)-1-(4-fluorophenyl)ethanol + NAD+ | - |
? | |
4'-iodoacetophenone + NADH + H+ | 31% of the activity with acetophenone | Candida viswanathii | (1S)-1-(4-iodophenyl)ethanol + NAD+ | - |
? | |
acetophenone + NADH + H+ | - |
Candida viswanathii | (1S)-1-phenylethanol + NAD+ | enantiomeric excess of more than 99% for (S)-configuration of product | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 29000, SDS-PAGE | Candida viswanathii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Candida viswanathii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
54% of maximum activity | Candida viswanathii |
75 | - |
61% of maximum activity | Candida viswanathii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
phosphate buffer, pH 7.0, 7 h, 80% residual activity | Candida viswanathii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Candida viswanathii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | more than 80% of maximum activity | Candida viswanathii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Candida viswanathii | |
NADH | - |
Candida viswanathii |