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Literature summary for 1.1.1.B20 extracted from

  • Li, L.; Zhang, L.; Li, K.; Wang, Y.; Gao, C.; Han, B.; Ma, C.; Xu, P.
    A newly isolated Bacillus licheniformis strain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical (2013), Biotechnol. Biofuels, 6, 123 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene bdh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetoin + NADH + H+ Bacillus licheniformis racemic mixture of (3S/3R)-acetoin meso-2,3-butanediol + NAD+
-
r
acetoin + NADH + H+ Bacillus licheniformis 10-1-A racemic mixture of (3S/3R)-acetoin meso-2,3-butanediol + NAD+
-
r
additional information Bacillus licheniformis stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A ?
-
?
additional information Bacillus licheniformis 10-1-A stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis X5F726 isolated from soil samples
-
Bacillus licheniformis 10-1-A X5F726 isolated from soil samples
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimization of culture conditions or strain 10-1-A and production of enzyme meso-2,3-BDH, overview Bacillus licheniformis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-acetoin + NADH + H+
-
Bacillus licheniformis meso-2,3-butanediol + NAD+
-
r
(3R)-acetoin + NADH + H+
-
Bacillus licheniformis 10-1-A meso-2,3-butanediol + NAD+
-
r
acetoin + NADH + H+ racemic mixture of (3S/3R)-acetoin Bacillus licheniformis meso-2,3-butanediol + NAD+
-
r
acetoin + NADH + H+ racemic mixture of (3S/3R)-acetoin Bacillus licheniformis 10-1-A meso-2,3-butanediol + NAD+
-
r
additional information stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A Bacillus licheniformis ?
-
?
additional information stereoisomeric composition analysis of 2,3-butanediol produced by strain 10-1-A using gas chromatography. Strain 10-1-A produces a mixture of (2R,3R)-2,3-butanediol and meso-2,3-butanediol with a ratio of nearly 1:1. As (3R)-acetoin is the major source of (2R,3R)-2,3-butanediol and meso-2,3-butanediol, a meso-butanediol dehydrogenase and a (2R,3R)-2,3-butanediol dehydrogenase are co-present in strain 10-1-A Bacillus licheniformis 10-1-A ?
-
?

Synonyms

Synonyms Comment Organism
BDH
-
Bacillus licheniformis
meso-BDH
-
Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus licheniformis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus licheniformis
NADH
-
Bacillus licheniformis

General Information

General Information Comment Organism
physiological function 2,3-butanediol dehydrogenase (BDH) catalyzes the interconversion between acetoin and 2,3-butanediol and is a key enzyme for 2,3-butanediol production Bacillus licheniformis