Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-alanine | 10 mM, activates | Arabidopsis thaliana | |
L-cysteine | 10 mM, activates | Arabidopsis thaliana | |
L-homocysteine | 10 mM, activates | Arabidopsis thaliana | |
L-homoserine | 10 mM, activates | Arabidopsis thaliana | |
L-methionine | 10 mM, activates | Arabidopsis thaliana | |
L-valine | 10 mM, activates | Arabidopsis thaliana | |
additional information | AtPGDH2 activity is not affected by threonine, valine, glycine, tryptophan, O-acetyl-L-serine, or cysteine | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-serine | 10 mM, inhibits AtPGDH3 at 55%; 10 mM, inhibits AtPGDH3 at 55% | Arabidopsis thaliana | |
additional information | L-serine (10 mM) does not inhibit AtPGDH2 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NAD+ | pH 9.0, 25°C | Arabidopsis thaliana | |
0.088 | - |
NAD+ | pH 9.0, 25°C | Arabidopsis thaliana | |
0.123 | - |
NAD+ | pH 9.0, 25°C | Arabidopsis thaliana | |
0.5 | - |
3-phospho-D-glycerate | pH 9.0, 25°C | Arabidopsis thaliana | |
0.77 | - |
3-phospho-D-glycerate | pH 9.0, 25°C | Arabidopsis thaliana | |
1.03 | - |
3-phospho-D-glycerate | pH 9.0, 25°C | Arabidopsis thaliana | |
3.7 | - |
2-oxoglutarate | pH 6.0, 25°C | Arabidopsis thaliana | |
3.9 | - |
oxaloacetate | pH 6.0, 25°C | Arabidopsis thaliana | |
7.6 | - |
2-oxoglutarate | pH 6.0, 25°C | Arabidopsis thaliana | |
10.7 | - |
2-oxoglutarate | pH 6.0, 25°C | Arabidopsis thaliana | |
11.2 | - |
oxaloacetate | pH 6.0, 25°C | Arabidopsis thaliana | |
14.1 | - |
oxaloacetate | pH 6.0, 25°C | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | Arabidopsis thaliana | first committed enzyme of the phosphorylated pathway of l-serine biosynthesis, regulated by negative feedback from L-serine in bacteria and plants | 3-phosphooxypyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O04130 | - |
- |
Arabidopsis thaliana | O49485 | - |
- |
Arabidopsis thaliana | Q9LT69 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + H+ | - |
Arabidopsis thaliana | D-2-hydroxyglutarate + NAD+ | - |
? | |
3-phospho-D-glycerate + NAD+ | - |
Arabidopsis thaliana | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
3-phospho-D-glycerate + NAD+ | first committed enzyme of the phosphorylated pathway of l-serine biosynthesis, regulated by negative feedback from L-serine in bacteria and plants | Arabidopsis thaliana | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
3-phosphooxypyruvate + NADH + H+ | - |
Arabidopsis thaliana | 3-phospho-D-glycerate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 60000, SDS-PAGE, AtPGDH2 forms an equilibrium of homooctamers and homotetramers | Arabidopsis thaliana |
homotetramer | 4 * 60000, SDS-PAGE | Arabidopsis thaliana |
homotetramer | 4 * 60000, SDS-PAGE, AtPGDH2 forms an equilibrium of homooctamers and homotetramers | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
3-phosphoglycerate dehydrogenase | - |
Arabidopsis thaliana |
At1g17745 | - |
Arabidopsis thaliana |
At3g19480 | - |
Arabidopsis thaliana |
At4g34200 | - |
Arabidopsis thaliana |
PGDH | - |
Arabidopsis thaliana |
PGDH1 | - |
Arabidopsis thaliana |
PGDH2 | - |
Arabidopsis thaliana |
PGDH3 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
2-oxoglutarate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.00018 | - |
2-oxoglutarate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.0002 | - |
2-oxoglutarate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.00051 | - |
oxaloacetate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.00077 | - |
oxaloacetate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.00135 | - |
oxaloacetate | pH 6.0, 25°C | Arabidopsis thaliana | |
15 | - |
NAD+ | pH 9.0, 25°C | Arabidopsis thaliana | |
15 | - |
3-phospho-D-glycerate | pH 9.0, 25°C | Arabidopsis thaliana | |
16 | - |
NAD+ | pH 9.0, 25°C | Arabidopsis thaliana | |
16 | - |
3-phospho-D-glycerate | pH 9.0, 25°C | Arabidopsis thaliana | |
17 | - |
NAD+ | pH 9.0, 25°C | Arabidopsis thaliana | |
17 | - |
3-phospho-D-glycerate | pH 9.0, 25°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
optimal pH for reduction of 2-oxoglutarate and oxaloacetate | Arabidopsis thaliana |
9 | - |
substrate: 3-phospho-D-glycerate, AtPGDH1 | Arabidopsis thaliana |
9.5 | - |
substrate: 3-phospho-D-glycerate, AtPGDH3 | Arabidopsis thaliana |
10 | - |
substrate: 3-phospho-D-glycerate, AtPGDH2 | Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | AtPGDH1 gene is coexpressed with genes of tryptophan biosynthesis. Activators of tryptophan biosynthesis MYB51 and MYB34 are involved in the expression of AtPGDH1 | up |
General Information | Comment | Organism |
---|---|---|
malfunction | tryptophan-derived metabolites such as indole acetic acid and indole glucosinolates are present at lower concentrations in AtPGDH1-silenced plants, demonstrating that serine used as a precursor for tryptophan biosynthesis is synthesized in the phosphorylated pathway, and that the involvement of AtPGDH1 is greatest among the three isoforms | Arabidopsis thaliana |
metabolism | first committed enzyme of the phosphorylated pathway of L-serine biosynthesis, regulated by negative feedback from L-serine in bacteria and plants | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000017 | - |
2-oxoglutarate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.000019 | - |
2-oxoglutarate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.000053 | - |
2-oxoglutarate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.000068 | - |
oxaloacetate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.000131 | - |
oxaloacetate | pH 6.0, 25°C | Arabidopsis thaliana | |
0.000907 | - |
oxaloacetate | pH 6.0, 25°C | Arabidopsis thaliana | |
16 | - |
3-phospho-D-glycerate | pH 9.0, 25°C | Arabidopsis thaliana | |
22 | - |
3-phospho-D-glycerate | pH 9.0, 25°C | Arabidopsis thaliana | |
30 | - |
3-phospho-D-glycerate | pH 9.0, 25°C | Arabidopsis thaliana | |
130 | - |
NAD+ | pH 9.0, 25°C | Arabidopsis thaliana | |
190 | - |
NAD+ | pH 9.0, 25°C | Arabidopsis thaliana | |
300 | - |
NAD+ | pH 9.0, 25°C | Arabidopsis thaliana |