Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-[6-(4-fluorophenyl)-2-sulfanylidene-1,6-dihydropyrimidin-5(2H)-ylidene](4-methylphenyl)methanolate | - |
Mycobacterium tuberculosis | |
N-[(4-methoxyphenyl)carbamoyl]-5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-aminide | - |
Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-acetolactate + NADPH + H+ | Mycobacterium tuberculosis | second enzyme in the branched-chain amino acid biosynthesis pathway | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
? | |
(S)-2-acetolactate + NADPH + H+ | Mycobacterium tuberculosis H37Rv | second enzyme in the branched-chain amino acid biosynthesis pathway | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WKJ7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WKJ7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-acetolactate + NADPH + H+ | - |
Mycobacterium tuberculosis | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
? | |
(2S)-2-acetolactate + NADPH + H+ | - |
Mycobacterium tuberculosis H37Rv | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
? | |
(S)-2-acetolactate + NADPH + H+ | second enzyme in the branched-chain amino acid biosynthesis pathway | Mycobacterium tuberculosis | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
? | |
(S)-2-acetolactate + NADPH + H+ | second enzyme in the branched-chain amino acid biosynthesis pathway | Mycobacterium tuberculosis H37Rv | (R)-2,3-dihydroxyisovalerate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KARI | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00306 | - |
N-[(4-methoxyphenyl)carbamoyl]-5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-aminide | pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.00371 | - |
(E)-[6-(4-fluorophenyl)-2-sulfanylidene-1,6-dihydropyrimidin-5(2H)-ylidene](4-methylphenyl)methanolate | pH 8.0, 25°C | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
drug target | the enzyme is present in bacteria but not in humans, making it an attractive proposition for drug discovery | Mycobacterium tuberculosis |
metabolism | second enzyme in the branched-chain amino acid biosynthesis pathway | Mycobacterium tuberculosis |