Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pyrococcus horikoshii |
expressed in Escherichia coli BL21(DE3) cells | Pyrococcus yayanosii |
expressed in Escherichia coli BL21(DE3) cells | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl | Pyrococcus horikoshii |
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl | Pyrococcus yayanosii |
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hydroxypyruvate | - |
Pyrococcus furiosus | |
Hydroxypyruvate | - |
Pyrococcus horikoshii | |
Hydroxypyruvate | - |
Pyrococcus yayanosii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Hydroxypyruvate | with NADPH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus horikoshii | |
0.07 | - |
Hydroxypyruvate | with NADH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus horikoshii | |
0.09 | - |
Hydroxypyruvate | with NADPH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus furiosus | |
0.14 | - |
Hydroxypyruvate | with NADH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus furiosus | |
0.165 | - |
Hydroxypyruvate | with NADPH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus yayanosii | |
0.41 | - |
Hydroxypyruvate | with NADH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus yayanosii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NADH + H+ | Pyrococcus horikoshii | - |
D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | Pyrococcus yayanosii | - |
D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | Pyrococcus furiosus | - |
D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | Pyrococcus yayanosii CH1 | - |
D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADPH + H+ | Pyrococcus horikoshii | - |
D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | Pyrococcus yayanosii | - |
D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | Pyrococcus furiosus | - |
D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | Pyrococcus yayanosii CH1 | - |
D-glycerate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U3Y2 | - |
- |
Pyrococcus horikoshii | - |
- |
- |
Pyrococcus yayanosii | F8AEA4 | - |
- |
Pyrococcus yayanosii CH1 | F8AEA4 | - |
- |
Purification (Comment) | Organism |
---|---|
Resource Q column chromatography and Superose 12 column chromatography | Pyrococcus horikoshii |
Resource Q column chromatography and Superose 12 column chromatography | Pyrococcus yayanosii |
Resource Q column chromatography and Superose 12 column chromatography | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NADH + H+ | - |
Pyrococcus horikoshii | D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | - |
Pyrococcus yayanosii | D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | - |
Pyrococcus furiosus | D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | - |
Pyrococcus yayanosii CH1 | D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADPH + H+ | - |
Pyrococcus horikoshii | D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | - |
Pyrococcus yayanosii | D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | - |
Pyrococcus furiosus | D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | - |
Pyrococcus yayanosii CH1 | D-glycerate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glyoxylate/hydroxypyruvate reductase | - |
Pyrococcus horikoshii |
glyoxylate/hydroxypyruvate reductase | - |
Pyrococcus yayanosii |
glyoxylate/hydroxypyruvate reductase | - |
Pyrococcus furiosus |
GRHPR | - |
Pyrococcus horikoshii |
GRHPR | - |
Pyrococcus yayanosii |
GRHPR | - |
Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
Hydroxypyruvate | with NADPH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus horikoshii | |
1.2 | - |
Hydroxypyruvate | with NADPH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus furiosus | |
4.5 | - |
Hydroxypyruvate | with NADH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus horikoshii | |
6.5 | - |
Hydroxypyruvate | with NADPH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus yayanosii | |
10 | - |
Hydroxypyruvate | with NADH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus furiosus | |
25 | - |
Hydroxypyruvate | with NADH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus yayanosii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the enzyme prefers NADH over NADPH | Pyrococcus horikoshii | |
NADH | the enzyme prefers NADH over NADPH | Pyrococcus yayanosii | |
NADH | the enzyme prefers NADH over NADPH | Pyrococcus furiosus | |
NADPH | the enzyme prefers NADH over NADPH | Pyrococcus horikoshii | |
NADPH | the enzyme prefers NADH over NADPH | Pyrococcus yayanosii | |
NADPH | the enzyme prefers NADH over NADPH | Pyrococcus furiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
Hydroxypyruvate | in the presence of NADH, at pH 8.0 and 50°C | Pyrococcus furiosus | |
0.76 | - |
Hydroxypyruvate | in the presence of NADH, at pH 8.0 and 50°C | Pyrococcus yayanosii | |
2 | - |
Hydroxypyruvate | in the presence of NADPH, at pH 8.0 and 50°C | Pyrococcus yayanosii | |
3.82 | - |
Hydroxypyruvate | in the presence of NADH, at pH 8.0 and 50°C | Pyrococcus horikoshii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
Hydroxypyruvate | with NADPH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus furiosus | |
30 | - |
Hydroxypyruvate | with NADPH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus horikoshii | |
40 | - |
Hydroxypyruvate | with NADPH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus yayanosii | |
60 | - |
Hydroxypyruvate | with NADH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus yayanosii | |
65 | - |
Hydroxypyruvate | with NADH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus horikoshii | |
75 | - |
Hydroxypyruvate | with NADH as cosubstrate, at pH 8.0 and 50°C | Pyrococcus furiosus |