Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Dunaliella salina |
gene GPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Dunaliella salina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Dunaliella salina | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76600 | - |
- |
Dunaliella salina |
100000 | - |
- |
Dunaliella salina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + NADH + H+ | Dunaliella salina | - |
sn-glycerol 3-phosphate + NAD+ | - |
r | |
dihydroxyacetone phosphate + NADH + H+ | Dunaliella salina 435 | - |
sn-glycerol 3-phosphate + NAD+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | Dunaliella salina | - |
glycerone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dunaliella salina | Q52ZA0 | - |
- |
Dunaliella salina | V9MH41 | - |
- |
Dunaliella salina 435 | Q52ZA0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + NADH + H+ | - |
Dunaliella salina | sn-glycerol 3-phosphate + NAD+ | - |
r | |
dihydroxyacetone phosphate + NADH + H+ | - |
Dunaliella salina 435 | sn-glycerol 3-phosphate + NAD+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | - |
Dunaliella salina | glycerone phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, recombinant enzyme, SDS-PAGE | Dunaliella salina |
? | x * 76600, about, sequence calculation, x* 100000, recombinant His-tagged enzyme, SDS-PAGE | Dunaliella salina |
? | x * 76600, calculated from amino acid sequence | Dunaliella salina |
Synonyms | Comment | Organism |
---|---|---|
G3PDH | - |
Dunaliella salina |
NAD+-dependent glycerol-3-phosphate dehydrogenase | - |
Dunaliella salina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Dunaliella salina | |
NADH | - |
Dunaliella salina |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dunaliella salina | calculated from amino acid sequence | - |
6.5 |
General Information | Comment | Organism |
---|---|---|
metabolism | glycerol-3-phosphate dehydrogenase (G3PDH) is a key enzyme in the pathway of glycerol synthesis, which converts dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate | Dunaliella salina |
physiological function | the glycerol-DHAP cycle pathway, which is driven by enzyme G3PDH, is considered as the rate-limiting enzyme to regulate the glycerol level under osmotic shocks | Dunaliella salina |