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Literature summary for 1.1.1.8 extracted from

  • Mizushima, D.; Iwata, H.; Ishimaki, Y.; Ogihara, J.; Kato, J.; Kasumi, T.
    Two glycerol 3-phosphate dehydrogenase isogenes from Candida versatilis SN-18 play an important role in glycerol biosynthesis under osmotic stress (2016), J. Biosci. Bioeng., 121, 523-529.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a gpd1DELTAgpd2DELTA double deletion mutant of Saccharomyces cerevisiae Wickerhamiella versatilis
expressed in Saccharomyces cerevisiae strain BY4741 Wickerhamiella versatilis
gene Cagpd1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant Wickerhamiella versatilis
gene Cagpd2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant Wickerhamiella versatilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Wickerhamiella versatilis
-
glycerone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+ Wickerhamiella versatilis
-
glycerone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ Wickerhamiella versatilis SN-18
-
glycerone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+ Wickerhamiella versatilis SN-18
-
glycerone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Wickerhamiella versatilis A0A140JW76
-
-
Wickerhamiella versatilis A0A140JW76 an osmotolerant yeast isolated from soil outside a food factory
-
Wickerhamiella versatilis A0A140JW77
-
-
Wickerhamiella versatilis A0A140JW77 an osmotolerant yeast isolated from soil outside a food factory
-
Wickerhamiella versatilis SN-18 A0A140JW76
-
-
Wickerhamiella versatilis SN-18 A0A140JW76 an osmotolerant yeast isolated from soil outside a food factory
-
Wickerhamiella versatilis SN-18 A0A140JW77
-
-
Wickerhamiella versatilis SN-18 A0A140JW77 an osmotolerant yeast isolated from soil outside a food factory
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.0009
-
crude enzyme extract, pH 8.5, 30┬░C Wickerhamiella versatilis
0.00147
-
crude enzyme extract, pH 8.5, 30┬░C Wickerhamiella versatilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with NADP+ Wickerhamiella versatilis ?
-
?
additional information no activity with NADP+ Wickerhamiella versatilis SN-18 ?
-
?
sn-glycerol 3-phosphate + NAD+
-
Wickerhamiella versatilis glycerone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+
-
Wickerhamiella versatilis glycerone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+
-
Wickerhamiella versatilis SN-18 glycerone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+
-
Wickerhamiella versatilis SN-18 glycerone phosphate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
Cagpd1
-
Wickerhamiella versatilis
Cagpd1 isoform Wickerhamiella versatilis
Cagpd1p
-
Wickerhamiella versatilis
Cagpd2
-
Wickerhamiella versatilis
Cagpd2 isoform Wickerhamiella versatilis
Cagpd2p
-
Wickerhamiella versatilis
glycerol 3-phosphate dehydrogenase
-
Wickerhamiella versatilis
GPD
-
Wickerhamiella versatilis
Gpd1p
-
Wickerhamiella versatilis
gpd2p
-
Wickerhamiella versatilis
NADP+-dependent glycerol 3-phosphate dehydrogenase
-
Wickerhamiella versatilis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Wickerhamiella versatilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Wickerhamiella versatilis

Cofactor

Cofactor Comment Organism Structure
additional information Cagpd1p is a NAD+-dependent GPD, no activity with NADP+ Wickerhamiella versatilis
additional information Cagpd2p is a NAD+-dependent GPD, no activity with NADP+ Wickerhamiella versatilis
NAD+
-
Wickerhamiella versatilis
NADH
-
Wickerhamiella versatilis

Expression

Organism Comment Expression
Wickerhamiella versatilis osmotic stress induces the isozyme up

General Information

General Information Comment Organism
evolution the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval Wickerhamiella versatilis
metabolism the enzyme is a key enzyme in the glycerol biosynthesis pathway Wickerhamiella versatilis
physiological function the enzyme is involved in osmotic stress response Wickerhamiella versatilis
physiological function two glycerol 3-phosphate dehydrogenase isozymes from Candida versatilis SN-18 play an important role in glycerol biosynthesis under osmotic stress. The enzyme is involved in osmotic stress response Wickerhamiella versatilis