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Literature summary for 1.1.1.8 extracted from

  • de la Roche, M.; Tessier, S.N.; Storey, K.B.
    Structural and functional properties of glycerol-3-phosphate dehydrogenase from a mammalian hibernator (2012), Protein J., 31, 109-119.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
NADH pH 7.5, 22┬░C, liver isozyme Cynomys ludovicianus
0.001
-
NADH pH 7.5, 5┬░C, liver isozyme Cynomys ludovicianus
0.001
-
NADH pH 7.5, 5┬░C, muscle isozyme Cynomys ludovicianus
0.003
-
NADH pH 7.5, 22┬░C, muscle isozyme Cynomys ludovicianus
0.045
-
NAD+ pH 8.5, 22┬░C, liver isozyme Cynomys ludovicianus
0.09
-
NAD+ pH 8.5, 5┬░C, liver isozyme Cynomys ludovicianus
0.143
-
NAD+ pH 8.5, 22┬░C, muscle isozyme Cynomys ludovicianus
0.154
-
glycerone phosphate pH 7.5, 5┬░C, muscle isozyme Cynomys ludovicianus
0.155
-
NAD+ pH 8.5, 5┬░C, muscle isozyme Cynomys ludovicianus
0.259
-
glycerone phosphate pH 7.5, 5┬░C, liver isozyme Cynomys ludovicianus
0.279
-
glycerone phosphate pH 7.5, 22┬░C, muscle isozyme Cynomys ludovicianus
0.352
-
glycerone phosphate pH 7.5, 22┬░C, liver isozyme Cynomys ludovicianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Cynomys ludovicianus
77000
-
gel filtration Cynomys ludovicianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Cynomys ludovicianus
-
glycerone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Cynomys ludovicianus
-
from Southeastern Wyoming
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 50fold and 176fold from liver and muscle, respectively, by anion exchange chromatography, gel filtration, and affinity chromatography Cynomys ludovicianus

Source Tissue

Source Tissue Comment Organism Textmining
liver liver isozyme Cynomys ludovicianus
-
skeletal muscle hibernator muscle, muscle isozyme Cynomys ludovicianus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
activities with the different isozymes at different temperatures, overview Cynomys ludovicianus
257
-
purified enzyme, from liver, pH 8.5, 25┬░C Cynomys ludovicianus
282
-
purified enzyme, from muscle, pH 8.5, 25┬░C Cynomys ludovicianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Cynomys ludovicianus glycerone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Cynomys ludovicianus

Synonyms

Synonyms Comment Organism
G3PDH
-
Cynomys ludovicianus
glycerol-3-phosphate dehydrogenase
-
Cynomys ludovicianus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Cynomys ludovicianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at, reduction reaction Cynomys ludovicianus
8.5
-
assay at, oxidation reaction Cynomys ludovicianus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cynomys ludovicianus
NADH
-
Cynomys ludovicianus

pI Value

Organism Comment pI Value Maximum pI Value
Cynomys ludovicianus isoelectric focusing
-
6.4

General Information

General Information Comment Organism
physiological function G3PDH is an important locus in fuel catabolism in hibernating species Cynomys ludovicianus