Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.8 extracted from

  • Berrada, W.; Naya, A.; Iddar, A.; Bourhim, N.
    Purification and characterization of cytosolic glycerol-3-phosphate dehydrogenase from skeletal muscle of jerboa (Jaculus orientalis) (2002), Mol. Cell. Biochem., 231, 117-127.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dichlorophenoxyacetic acid
-
Jaculus orientalis
Cd2+ 50% inhibition at 8.33 mM; 50% inhibition at 8.3 mM Jaculus orientalis
Cu2+ 50% inhibition at 12.96 mM; 50% inhibition at 13.0 mM Jaculus orientalis
K+ 50% inhibition at 40.66 mM; 50% inhibition at 40.7 mM Jaculus orientalis
Mn2+ 50% inhibition at 36.6 mM Jaculus orientalis
additional information not inhibitory: CaCl2, MgCl2, NaCl Jaculus orientalis
N-ethylmaleimide 20 mM, complete inhibition; 20 mM, complete loss of activity Jaculus orientalis
Ni2+ 50% inhibition at 31.66 mM; 50% inhibition at 31.7 mM Jaculus orientalis
Zn2+ 50% inhibition at 5.66 mM; 50% inhibition at 5.7 mM Jaculus orientalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
NADH pH 7.6, 25┬░C Jaculus orientalis
0.137
-
glycerone phosphate pH 7.6, 25┬░C Jaculus orientalis
0.316
-
NAD+ pH 9.0, 25┬░C Jaculus orientalis
0.468
-
glycerol-3-phosphate pH 9.0, 25┬░C Jaculus orientalis
32.4
-
NADH pH 9.0, 25┬░C Jaculus orientalis
137
-
glycerone phosphate pH 9.0, 25┬░C Jaculus orientalis
316
-
NAD+ pH 7.6, 25┬░C Jaculus orientalis
469
-
sn-glycerol 3-phosphate pH 7.6, 25┬░C Jaculus orientalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Jaculus orientalis 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
2 * 33000, SDS-PAGE Jaculus orientalis
72000
-
gel filtration Jaculus orientalis
72000
-
PAGE Jaculus orientalis

Organism

Organism UniProt Comment Textmining
Jaculus orientalis
-
-
-
Jaculus orientalis
-
jerboa
-

Purification (Commentary)

Purification (Comment) Organism
-
Jaculus orientalis

Reaction

Reaction Comment Organism Reaction ID
sn-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor ordered bi-bi mechanism Jaculus orientalis
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ ordered bi-bi mechanism Jaculus orientalis

Source Tissue

Source Tissue Comment Organism Textmining
muscle skeletal muscle Jaculus orientalis
-
skeletal muscle
-
Jaculus orientalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
53.6
-
pH 7.6, 25┬░C Jaculus orientalis
55.6
-
pH 9.0, 25┬░C Jaculus orientalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol-3-phosphate + NAD+
-
Jaculus orientalis glycerone phosphate + NADH
-
r
glycerone phosphate + NADH
-
Jaculus orientalis glycerol-3-phosphate + NAD+
-
r
glycerone phosphate + NADH
-
Jaculus orientalis sn-glycerol 3-phosphate + NAD+
-
r
glycerone phosphate + NADH + H+
-
Jaculus orientalis sn-glycerol 3-phosphate + NAD+
-
r
sn-glycerol 3-phosphate + NAD+
-
Jaculus orientalis glycerone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE Jaculus orientalis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
35
-
-
Jaculus orientalis
35
-
oxidation of sn-glycerol-3-phosphate Jaculus orientalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
reduction reaction Jaculus orientalis
7.7
-
reduction of dihydroxyacetone phosphate Jaculus orientalis
9
-
oxidation reaction Jaculus orientalis
9
-
oxidation of sn-glycerol-3-phosphate Jaculus orientalis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Jaculus orientalis
NADH
-
Jaculus orientalis