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Literature summary for 1.1.1.8 extracted from

  • Agboola, F.K.; Thomson, A.; Afolayan, A.
    Isolation and properties of cytoplasmic alpha-glycerol 3-phosphate dehydrogenase from the pectoral muscle of the fruit bat, Eidolon helvum (2003), J. Biochem. Mol. Biol., 36, 159-166.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
NADH pH 7.5, 25┬░C Eidolon helvum
0.2
-
NAD+ pH 10.0, 25┬░C Eidolon helvum
0.26
-
glycerone phosphate pH 7.5, 25┬░C Eidolon helvum
0.65
-
NAD+ pH 7.5, 25┬░C Eidolon helvum
2.3
-
L-glycerol-3-phosphate pH 10.0, 25┬░C Eidolon helvum
3.9
-
L-glycerol-3-phosphate pH 7.5, 25┬░C Eidolon helvum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Eidolon helvum 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35700
-
2 * 35700, SDS-PAGE Eidolon helvum
59500
-
gel filtration Eidolon helvum

Organism

Organism UniProt Comment Textmining
Eidolon helvum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Eidolon helvum

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Eidolon helvum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
120
-
pH 7.5, 25┬░C Eidolon helvum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerone phosphate + NADH
-
Eidolon helvum L-glycerol-3-phosphate + NAD+
-
r
L-glycerol-3-phosphate + NAD+
-
Eidolon helvum glycerone phosphate + NADH
-
r

Subunits

Subunits Comment Organism
dimer 2 * 35700, SDS-PAGE Eidolon helvum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51
-
L-glycerol-3-phosphate pH 7.5, 25┬░C Eidolon helvum
78
-
L-glycerol-3-phosphate pH 10.0, 25┬░C Eidolon helvum
19000
-
glycerone phosphate pH 7.5, 25┬░C Eidolon helvum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Eidolon helvum
NADH
-
Eidolon helvum