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Literature summary for 1.1.1.8 extracted from

  • Koekemoer, T.C.; Litthauer, D.; Oelofsen, W.
    Isolation and characterization of adipose tissue glycerol-3-phasphate dehydrogenase (1995), Int. J. Biochem. Cell Biol., 27, 625-632.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glycerol competitive activator with respect to dihydroxyacetone phosphate above 30 mM Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide effective inhibitor Homo sapiens
iodoacetate 50 mM, incubated for 60 min Homo sapiens
NADPH
-
Homo sapiens
p-chloromercuribenzoate effective inhibitor Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, SDS-PAGE Homo sapiens
74000
-
electrophoresis in a non-denaturing system Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Homo sapiens
-
dihydroxyacetone phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
250
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20Ā°C, 50% glycerol, retains activity for several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Homo sapiens dihydroxyacetone phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
dihydroxyacetone phosphate reduction Homo sapiens
9
-
glycerol-3-phosphate oxidation Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Homo sapiens
NAD+
-
Homo sapiens
NADH
-
Homo sapiens