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Literature summary for 1.1.1.8 extracted from

  • Kohl, L.; Drmota, T.; Do Thi, C.D.; Callens, M.; Van Beeumen, J.; Opperdoes, F.R.; Michels, P.A.M.
    Clonig and characterization of the NAD-linked glycerol-3-phosphate dehydrogenase of Trypanosoma brucei brucei and Leishmania mexicana mexicana and expression of the trypanosome enzyme in Escherichia coli (1996), Mol. Biochem. Parasitol., 76, 159-173.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant in Escherichia coli Trypanosoma brucei
recombinant in Escherichia coli Leishmania mexicana

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
Trypanosoma brucei 20015
-
glycosome
-
Leishmania mexicana 20015
-
mitochondrion determined by isopycnic gradient centrifugation Leishmania mexicana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37651
-
x * 37651, calculated by cDNA-sequence Trypanosoma brucei
39140
-
x * 39140, calculated by cDNA-sequence Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Trypanosoma brucei
-
dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+ Leishmania mexicana
-
dihydroxyacetone phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Leishmania mexicana
-
strain Leishmania mexicana mexicana
-
Trypanosoma brucei
-
strain Trypanosoma brucei brucei
-

Purification (Commentary)

Purification (Comment) Organism
-
Trypanosoma brucei
-
Leishmania mexicana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
80
-
estimated Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Trypanosoma brucei dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+
-
Leishmania mexicana dihydroxyacetone phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 37651, calculated by cDNA-sequence Trypanosoma brucei
? x * 39140, calculated by cDNA-sequence Leishmania mexicana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Trypanosoma brucei
NADH
-
Trypanosoma brucei