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Literature summary for 1.1.1.8 extracted from

  • Marche, S.; Michels, P.A.M.; Opperdoes, F.R.
    Comparative study of Leishmania mexicana and trypanosoma brucei NAD-dependent glycerol-3-phosphate dehydrogenase (2000), Mol. Biochem. Parasitol., 106, 83-91.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant in Escherichia coli Leishmania mexicana

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxy-1,2,3-nonadecanetricarboxylic acid trypanocidal drug, IC50 0.00055 mM Leishmania mexicana
2-hydroxy-1,2,3-nonadecanetricarboxylic acid IC50 0.0011 mM Trypanosoma brucei
dihydroxyacetone phosphate substrate inhibition at 0.2 mM Leishmania mexicana
dihydroxyacetone phosphate substrate inhibition at 0.2 mM Trypanosoma brucei
Melarsen oxide active principle of the trypanocidal drug melarsoprol and cymelarsen Leishmania mexicana
Melarsen oxide active principle of the trypanocidal drug melarsoprol and cymelarsen; cymelarsen, IC50 0.0015-0.005 mM Trypanosoma brucei
NaCl strongly inhibits, 50% activity at 250 mM NaCl Leishmania mexicana
NaCl strongly inhibits, 50% activity at 250 mM NaCl Trypanosoma brucei
NADPH inhibits in presence of saturing concentration of NADH and dihydroxyacetone phosphate Leishmania mexicana
NADPH inhibits in presence of saturing concentration of NADH and dihydroxyacetone phosphate Trypanosoma brucei
Nucleic acids strongly inhibit Leishmania mexicana
p-chloromercuribenzoate 0.1 mM, reversed by thiols, e.g. dithiothreitol Trypanosoma brucei
suramin trypanocidal drug, potent inhibitor, IC50 0.0002 mM Leishmania mexicana
suramin IC50 0.00044 mM Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADH
-
Trypanosoma brucei
0.024
-
NADH
-
Leishmania mexicana
0.1
-
dihydroxyacetone phosphate
-
Trypanosoma brucei
0.12
-
dihydroxyacetone phosphate
-
Leishmania mexicana
0.32
-
NAD+
-
Trypanosoma brucei
0.42
-
NAD+
-
Leishmania mexicana
1.7
-
glycerol-3-phosphate
-
Leishmania mexicana
2.25
-
glycerol-3-phosphate
-
Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
Trypanosoma brucei 20015
-
glycosome
-
Leishmania mexicana 20015
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Trypanosoma brucei
-
dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+ Leishmania mexicana
-
dihydroxyacetone phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Leishmania mexicana
-
-
-
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Trypanosoma brucei
-
Leishmania mexicana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
154
-
-
Leishmania mexicana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Trypanosoma brucei dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+
-
Leishmania mexicana dihydroxyacetone phosphate + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Leishmania mexicana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
glycerol-3-phosphate formation Trypanosoma brucei
6.5
-
glycerol-3-phosphate formation Leishmania mexicana
9.6
-
dihydroxyacetone phosphate formation Trypanosoma brucei
9.6
-
dihydroxyacetone phosphate formation Leishmania mexicana

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 highest activity, glycerol-3-phosphate formation Trypanosoma brucei
5 7.5 highest activity, glycerol-3-phosphate formation Leishmania mexicana

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 under diluted conditions Trypanosoma brucei
5 9 under diluted conditions Leishmania mexicana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Trypanosoma brucei
NADH
-
Trypanosoma brucei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0002
-
trypanocidal drug, potent inhibitor, IC50 0.0002 mM Leishmania mexicana suramin
0.00044
-
IC50 0.00044 mM Trypanosoma brucei suramin
0.00055
-
trypanocidal drug, IC50 0.00055 mM Leishmania mexicana 2-hydroxy-1,2,3-nonadecanetricarboxylic acid
0.0011
-
IC50 0.0011 mM Trypanosoma brucei 2-hydroxy-1,2,3-nonadecanetricarboxylic acid
0.0015 0.005 cymelarsen, IC50 0.0015-0.005 mM Trypanosoma brucei Melarsen oxide