Cloned (Comment) | Organism |
---|---|
recombinant in Escherichia coli | Leishmania mexicana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxy-1,2,3-nonadecanetricarboxylic acid | trypanocidal drug, IC50 0.00055 mM | Leishmania mexicana | |
2-hydroxy-1,2,3-nonadecanetricarboxylic acid | IC50 0.0011 mM | Trypanosoma brucei | |
dihydroxyacetone phosphate | substrate inhibition at 0.2 mM | Leishmania mexicana | |
dihydroxyacetone phosphate | substrate inhibition at 0.2 mM | Trypanosoma brucei | |
Melarsen oxide | active principle of the trypanocidal drug melarsoprol and cymelarsen | Leishmania mexicana | |
Melarsen oxide | active principle of the trypanocidal drug melarsoprol and cymelarsen; cymelarsen, IC50 0.0015-0.005 mM | Trypanosoma brucei | |
NaCl | strongly inhibits, 50% activity at 250 mM NaCl | Leishmania mexicana | |
NaCl | strongly inhibits, 50% activity at 250 mM NaCl | Trypanosoma brucei | |
NADPH | inhibits in presence of saturing concentration of NADH and dihydroxyacetone phosphate | Leishmania mexicana | |
NADPH | inhibits in presence of saturing concentration of NADH and dihydroxyacetone phosphate | Trypanosoma brucei | |
Nucleic acids | strongly inhibit | Leishmania mexicana | |
p-chloromercuribenzoate | 0.1 mM, reversed by thiols, e.g. dithiothreitol | Trypanosoma brucei | |
suramin | trypanocidal drug, potent inhibitor, IC50 0.0002 mM | Leishmania mexicana | |
suramin | IC50 0.00044 mM | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | - |
Trypanosoma brucei | |
0.024 | - |
NADH | - |
Leishmania mexicana | |
0.1 | - |
dihydroxyacetone phosphate | - |
Trypanosoma brucei | |
0.12 | - |
dihydroxyacetone phosphate | - |
Leishmania mexicana | |
0.32 | - |
NAD+ | - |
Trypanosoma brucei | |
0.42 | - |
NAD+ | - |
Leishmania mexicana | |
1.7 | - |
glycerol-3-phosphate | - |
Leishmania mexicana | |
2.25 | - |
glycerol-3-phosphate | - |
Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma brucei | 20015 | - |
glycosome | - |
Leishmania mexicana | 20015 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Leishmania mexicana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Trypanosoma brucei | - |
dihydroxyacetone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | Leishmania mexicana | - |
dihydroxyacetone phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | - |
- |
- |
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Trypanosoma brucei |
- |
Leishmania mexicana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
154 | - |
- |
Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Trypanosoma brucei | dihydroxyacetone phosphate + NADH + H+ | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Leishmania mexicana | dihydroxyacetone phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Leishmania mexicana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
glycerol-3-phosphate formation | Trypanosoma brucei |
6.5 | - |
glycerol-3-phosphate formation | Leishmania mexicana |
9.6 | - |
dihydroxyacetone phosphate formation | Trypanosoma brucei |
9.6 | - |
dihydroxyacetone phosphate formation | Leishmania mexicana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | highest activity, glycerol-3-phosphate formation | Trypanosoma brucei |
5 | 7.5 | highest activity, glycerol-3-phosphate formation | Leishmania mexicana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | under diluted conditions | Trypanosoma brucei |
5 | 9 | under diluted conditions | Leishmania mexicana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Trypanosoma brucei | |
NADH | - |
Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0002 | - |
trypanocidal drug, potent inhibitor, IC50 0.0002 mM | Leishmania mexicana | suramin | |
0.00044 | - |
IC50 0.00044 mM | Trypanosoma brucei | suramin | |
0.00055 | - |
trypanocidal drug, IC50 0.00055 mM | Leishmania mexicana | 2-hydroxy-1,2,3-nonadecanetricarboxylic acid | |
0.0011 | - |
IC50 0.0011 mM | Trypanosoma brucei | 2-hydroxy-1,2,3-nonadecanetricarboxylic acid | |
0.0015 | 0.005 | cymelarsen, IC50 0.0015-0.005 mM | Trypanosoma brucei | Melarsen oxide |