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Literature summary for 1.1.1.8 extracted from

  • Nilsson, A.; Adler, L.
    Purification and characterization of glycerol-3-phosphate dehydrogenase (NAD+) in the salt-tolerant yeast Debaryomyces hansenii (1990), Biochim. Biophys. Acta, 1034, 180-185.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dipotassium malate slight activation at low ionic strength Debaryomyces hansenii
potassium glutamate up to 0.1 M, activation, high activity also at high ionic strength Debaryomyces hansenii

General Stability

General Stability Organism
20% polyethylene glycol stabilizes during purification Debaryomyces hansenii
substrates stabilize dilute preparation Debaryomyces hansenii

Inhibitors

Inhibitors Comment Organism Structure
glycerol slight inhibition Debaryomyces hansenii
KCl inactivation Debaryomyces hansenii
malate at high concentration Debaryomyces hansenii
NaCl inactivation Debaryomyces hansenii
NAD+ competitive inhibitor to NADH at physiological concentration Debaryomyces hansenii
SO42- at high concentration, MgSO4 most inhibitory Debaryomyces hansenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0066
-
NADH standard deviation 0.003 mM Debaryomyces hansenii
0.13
-
dihydroxyacetone phosphate standard deviation 0.03 mM Debaryomyces hansenii
0.3
-
NAD+ standard deviation 0.06 mM Debaryomyces hansenii
1.2
-
DL-glycerol-3-phosphate standard deviation 0.5 mM Debaryomyces hansenii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 0.1-0.3 M, chloride salts become strongly inhibitory at higher concentrations, replacing K+ with Na+ or NH4+yields similar results Debaryomyces hansenii
MgCl2 0.1-0.3 M Debaryomyces hansenii
MgSO4 0.1-0.3 M, slight activation at low ionic strength, most inhibitory at higher concentration Debaryomyces hansenii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE Debaryomyces hansenii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Debaryomyces hansenii
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Debaryomyces hansenii
-
van Rij strain 26, salt-tolerant
-

Purification (Commentary)

Purification (Comment) Organism
2 isozymes Debaryomyces hansenii

Reaction

Reaction Comment Organism Reaction ID
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ ordered ternary-complex mechanism, NADH binds first Debaryomyces hansenii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
200
-
-
Debaryomyces hansenii

Storage Stability

Storage Stability Organism
0┬░C, 30 mM Tris-H2SO4, 2.5 mM Na2EDTA, 5 mM dithiothreitol, 0,05% NaN3 (pH 8, 4┬░C), 0.1 mM phenylmethylsulfonylfluoride, 15-17.5% w/v poly(ethylene glycol) 4000, after six days loss of activity insignificant Debaryomyces hansenii
0┬░C, crude extract stable for 6 days in presence of polyethylene glycol Debaryomyces hansenii
4┬░C, stable Debaryomyces hansenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information glyceraldehyde-3-phosphate Debaryomyces hansenii ?
-
?
additional information no substrates: dihydroxyacetone Debaryomyces hansenii ?
-
?
sn-glycerol 3-phosphate + NAD+
-
Debaryomyces hansenii dihydroxyacetone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Debaryomyces hansenii

pH Range

pH Minimum pH Maximum Comment Organism
7.9 8.2 maximum activity in pH interval 7.9-8.2 Debaryomyces hansenii

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Debaryomyces hansenii
NAD+
-
Debaryomyces hansenii
NADH
-
Debaryomyces hansenii