Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | slight activation | Spinacia oleracea | |
bovine serum albumin | activity increased | Spinacia oleracea | |
dihydrolipoic acid | stimulation of chloroplastic form, 147% of enzyme activity | Spinacia oleracea | |
dithiothreitol | stimulation of chloroplastic form, 150% of enzyme activity | Spinacia oleracea | |
fructose 2,6-bisphosphate | 0.025 mM maximum stimulation of 2.3fold of cytosolic form, not chloroplastic form, no stimulation by fructose-1,6-bisphosphate | Spinacia oleracea | |
phosphate | around 5 mM, activation, chloroplastic form | Spinacia oleracea | |
phosphogluconate | 0.005 mM slightly stimulates chloroplastic form | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | inhibition of chloroplastic and cytosolic form | Spinacia oleracea | |
2-amino-2-hydroxymethylpropane-1,3-diol | i.e. Tris | Spinacia oleracea | |
glycerol phosphate | 0.025 mM, strong inhibition of chloroplastic and cytosolic form, endproduct inhibition | Spinacia oleracea | |
L-glycerol 3-phosphate | over 0.025 mM, strong inhibitor of cytosolic and chloroplastic enzyme | Spinacia oleracea | |
Large peptide factor | chloroplast enzyme | Spinacia oleracea | |
linoleic acid | 0.003 mM, 40% inhibition of chloroplastic form and 43% inhibition of cytosolic form | Spinacia oleracea | |
additional information | cyclic-AMP | Spinacia oleracea | |
octyl glucose | 0.003 mM, 41% inhibition of chloroplastic form and 61% inhibition of cytosolic form | Spinacia oleracea | |
palmitic acid | 0.003 mM, 40% inhibition of chloroplastic form and 43% inhibition of cytosolic form | Spinacia oleracea | |
phenylmethyl sulfonyl fluoride | 0.5 mM 50% inhibition of cytosolic form, little effect on chloroplastic form | Spinacia oleracea | |
phosphate | slight inhibition of cytosolic form, at higher concentrations above 20-30 mM both forms are inhibited | Spinacia oleracea | |
phosphatidyl choline | 0.003 mM, 42% inhibition of chloroplastic form and 43% inhibition of cytosolic form | Spinacia oleracea | |
phosphogluconate | cytosolic isozyme | Spinacia oleracea | |
reduced thioredoxin | stimulation of chloroplastic form | Spinacia oleracea | |
sedoheptulose 1,7-bisphosphate | 0.5 mM, chloroplastic and cytosolic form inhibited by 50% | Spinacia oleracea | |
Small peptide factor | cytosolic enzyme | Spinacia oleracea | |
Thylakoid fraction | 0.02 mM 97% inhibition | Spinacia oleracea | |
Triton X-100 | 0.006 mM, 50% inhibition of chloroplastic form and 52% inhibition of cytosolic form | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | stroma, soluble | Spinacia oleracea | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Spinacia oleracea | - |
dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
spinach, cv Long Standing Bloomsdale | - |
Purification (Comment) | Organism |
---|---|
2 isozymes | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Spinacia oleracea | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Spinacia oleracea |