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Literature summary for 1.1.1.8 extracted from

  • Merkel, J.R.; Straume, M.; Sajer, S.A.; Hopfer, R.L.
    Purification and some properties of sn-glycerol-3-phosphate dehydrogenase from Saccharomyces cerevisiae (1982), Anal. Biochem., 122, 180-185.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cl- cations, i.e. H+, K+, Na+ associated with Cl- do not affect the reduction of dihydroxyacetone phosphate Saccharomyces cerevisiae
additional information
-
Saccharomyces cerevisiae
NaCl inactivates irreversibly Saccharomyces cerevisiae
phosphate
-
Saccharomyces cerevisiae
TEA-buffer
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, gel filtration, estimated from elution volume on sepharose 6B Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Saccharomyces cerevisiae reverse reaction favoured direction dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
158
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20-0Ā°C, stable in 75% ammonium sulfate Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Saccharomyces cerevisiae dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ reverse reaction favoured direction Saccharomyces cerevisiae dihydroxyacetone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 31000, gel filtration, estimated from elution volume on sepharose 6B Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.8 7.2 activity falls off rapidly as pH is increased Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Saccharomyces cerevisiae
NAD+
-
Saccharomyces cerevisiae
NADH
-
Saccharomyces cerevisiae