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Literature summary for 1.1.1.8 extracted from

  • Niesel, D.W.; Bewley, G.C.; Lee, C.Y.; Armstrong, F.B.
    sn-Glycerol-3-phospate dehydrogenase (soluble) from Drosophila melanogaster (1982), Methods Enzymol., 89, 296-301.
No PubMed abstract available

General Stability

General Stability Organism
NADH stabilizes Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km values of isozyme GPDH-3 Drosophila melanogaster
0.078
-
NADH GPDH-1 Drosophila melanogaster
0.38
-
NAD+ GPDH-1 Drosophila melanogaster
0.52
-
dihydroxyacetone phosphate GPDH-1 Drosophila melanogaster
0.59
-
glycerol 3-phosphate GPDH-1 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Drosophila melanogaster
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
fruit fly, Samarkand wild-type
-

Purification (Commentary)

Purification (Comment) Organism
3 isozymes, product of the same gene mapped to left arm of chromosome II, isozyme 1: flight muscle, isozyme 3: larval and adult fat body, isozyme 2: heterodimer of 1 and 3 Drosophila melanogaster
affinity chromatography Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
muscle thoracic flight muscle Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
156
-
isozyme GPDH-3 Drosophila melanogaster
179.8
-
isozyme GPDH-1 Drosophila melanogaster

Storage Stability

Storage Stability Organism
5Ā°C, isozyme 1: stable for over a month in 10 mM sodium phosphate buffer, pH 6.5, with added EDTA, dithiothreitol and NADH, isozyme 3: 50% loss of activity under the same conditions Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Drosophila melanogaster dihydroxyacetone phosphate + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
reduction of dihydroxyacetone-P, isozyme GPDH-1 Drosophila melanogaster
7.4
-
reduction of dihydroxyacetone-phosphate, isozyme GPDH-3 Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Drosophila melanogaster
NADH
-
Drosophila melanogaster