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Literature summary for 1.1.1.8 extracted from

  • Niesel, D.W.; Bewley, G.C.; Miller, S.G.; Armstrong, F.B.; Lee, C.Y.
    Purification and structural analysis of the soluble sn-glycerol-3-phosphate dehydrogenase isozymes in Drosophila melanogaster (1980), J. Biol. Chem., 255, 4073-4080.
    View publication on PubMed

General Stability

General Stability Organism
dithiothreitol stabilizes Drosophila melanogaster
phenylmethane-sulfonyl fluoride stabilizes during purification Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
ATP weak inhibitor of both isozymes, i.e. GPDH-1 and -3 Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, SDS-PAGE, confirmed by amino acid composition Drosophila melanogaster
66000
-
gel filtration Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Drosophila melanogaster
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3 isozymes in adults and 1 in larvae, distinct in isoelectric focusing Drosophila melanogaster
affinity chromatography Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
larva late third instar Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
156
-
isozyme GPDH-3 Drosophila melanogaster
179.8
-
isozyme GPDH-1 Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Drosophila melanogaster dihydroxyacetone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE, confirmed by amino acid composition Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Drosophila melanogaster
NADH
-
Drosophila melanogaster