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Literature summary for 1.1.1.8 extracted from

  • Kornbluth, R.; Tracy, P.S.; Fondy, T.P.
    Isoenzymic forms of NAD-linked glycerol-3-phosphate dehydrogenase from rabbit brain (1979), Biochim. Biophys. Acta, 568, 273-286.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km values of isozyme I5.9 Oryctolagus cuniculus
0.01
-
NAD+ I6.5 Oryctolagus cuniculus
0.01
-
NADH I6.5 Oryctolagus cuniculus
0.2
-
dihydroxyacetone phosphate I6.5 Oryctolagus cuniculus
0.3
-
DL-glycerol-3-phosphate I6.5 Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Oryctolagus cuniculus
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 isozymes with distinct isoelectric points in brain Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Oryctolagus cuniculus
-
muscle heart muscle Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
77
-
isozyme I6.5, after affinity chromatography, after preparative isoelectric-focusing: 175 units/mg Oryctolagus cuniculus
162
-
isozyme I5.9 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Oryctolagus cuniculus dihydroxyacetone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Oryctolagus cuniculus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
48
-
t1/2: 7.5 min, isozyme I6.5 Oryctolagus cuniculus
48
-
t1/2: 2 min, brain isozyme I5.9 Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Oryctolagus cuniculus
NADH
-
Oryctolagus cuniculus