Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.8 extracted from

  • Santora, G.T.; Gee, R.; Tolbert, N.E.
    Isolation of a sn-glycerol 3-phosphate: NAD oxidoreductase from spinach leaves (1979), Arch. Biochem. Biophys., 196, 403-411.
    View publication on PubMed

General Stability

General Stability Organism
freezing inactivates completely, ammonium sulfate prevents inactivation Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
NADH at high concentration Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADH
-
Spinacia oleracea
0.19
-
NAD+
-
Spinacia oleracea
0.3
-
dihydroxyacetone phosphate 0.30-0.35 mM Spinacia oleracea
1.6
-
glycerol phosphate
-
Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Spinacia oleracea 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63500
-
gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Spinacia oleracea oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
37.5
-
-
Spinacia oleracea

Storage Stability

Storage Stability Organism
-20┬░C, stable as (NH4)2SO4-precipitate Spinacia oleracea
4┬░C, purified and dilute preparation stable for several days in presence of NADH Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information glycerol Spinacia oleracea ?
-
?
additional information glyceraldehyde-3-phosphate Spinacia oleracea ?
-
?
additional information DL-glyceraldehyde, phosphohydroxypyruvate Spinacia oleracea ?
-
?
additional information fructose-6-phosphate, fructose-1,6-bisphosphate Spinacia oleracea ?
-
?
additional information no substrates: dihydroxyacetone Spinacia oleracea ?
-
?
sn-glycerol 3-phosphate + NAD+
-
Spinacia oleracea dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH Spinacia oleracea dihydroxyacetone phosphate + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
reduction of dihydroxyacetone-P Spinacia oleracea
9.5
-
oxidation of glycerol-phosphate Spinacia oleracea

pH Range

pH Minimum pH Maximum Comment Organism
5 8.1 about half-maximal activity at pH 5.0 and 8.1, reduction of dihydroxyacetone phosphate Spinacia oleracea
5.8 7.2 reduction of dihydroxyacetone phosphate occurs over a broad range between 5.8 to 7.2 Spinacia oleracea
8.6 10 about half-maximal activity at pH 8.6 and 10.0, oxidation of glycerol 3-phosphate Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Spinacia oleracea
additional information not NADP+ Spinacia oleracea
NAD+
-
Spinacia oleracea
NADH
-
Spinacia oleracea