General Stability | Organism |
---|---|
freezing inactivates completely, ammonium sulfate prevents inactivation | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | at high concentration | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | - |
Spinacia oleracea | |
0.19 | - |
NAD+ | - |
Spinacia oleracea | |
0.3 | - |
dihydroxyacetone phosphate | 0.30-0.35 mM | Spinacia oleracea | |
1.6 | - |
glycerol phosphate | - |
Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63500 | - |
gel filtration | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Spinacia oleracea | oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.5 | - |
- |
Spinacia oleracea |
Storage Stability | Organism |
---|---|
-20°C, stable as (NH4)2SO4-precipitate | Spinacia oleracea |
4°C, purified and dilute preparation stable for several days in presence of NADH | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | glycerol | Spinacia oleracea | ? | - |
? | |
additional information | glyceraldehyde-3-phosphate | Spinacia oleracea | ? | - |
? | |
additional information | DL-glyceraldehyde, phosphohydroxypyruvate | Spinacia oleracea | ? | - |
? | |
additional information | fructose-6-phosphate, fructose-1,6-bisphosphate | Spinacia oleracea | ? | - |
? | |
additional information | no substrates: dihydroxyacetone | Spinacia oleracea | ? | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Spinacia oleracea | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH | Spinacia oleracea | dihydroxyacetone phosphate + NADH + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
reduction of dihydroxyacetone-P | Spinacia oleracea |
9.5 | - |
oxidation of glycerol-phosphate | Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.1 | about half-maximal activity at pH 5.0 and 8.1, reduction of dihydroxyacetone phosphate | Spinacia oleracea |
5.8 | 7.2 | reduction of dihydroxyacetone phosphate occurs over a broad range between 5.8 to 7.2 | Spinacia oleracea |
8.6 | 10 | about half-maximal activity at pH 8.6 and 10.0, oxidation of glycerol 3-phosphate | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Spinacia oleracea | |
additional information | not NADP+ | Spinacia oleracea | |
NAD+ | - |
Spinacia oleracea | |
NADH | - |
Spinacia oleracea |