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Literature summary for 1.1.1.8 extracted from

  • Fernandez-Sousa, J.M.; Gavilanes, J.G.; Municio, A.M.; Perez-Aranda, A.
    L-glycerol-3-phosphate dehydrogenase from the insect Ceratitis capitata. Purfication, physicochemical and enzymic properties (1977), Biochim. Biophys. Acta, 481, 6-24.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification Ceratitis capitata

Inhibitors

Inhibitors Comment Organism Structure
additional information high ionic strength above 0.03 M Ceratitis capitata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
NADH
-
Ceratitis capitata
0.26
-
NAD+
-
Ceratitis capitata
0.33
-
dihydroxyacetone phosphate
-
Ceratitis capitata
0.74
-
sn-glycerol 3-phosphate
-
Ceratitis capitata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
2 * 33500, SDS-PAGE Ceratitis capitata
72500
-
69000-76000, gel filtration Ceratitis capitata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Ceratitis capitata
-
dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ Ceratitis capitata Wiedemann
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Ceratitis capitata
-
mediterranean fruit fly
-
Ceratitis capitata Wiedemann
-
mediterranean fruit fly
-

Purification (Commentary)

Purification (Comment) Organism
-
Ceratitis capitata

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
198.9
-
-
Ceratitis capitata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Ceratitis capitata dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+
-
Ceratitis capitata Wiedemann dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+
-
Ceratitis capitata glycerone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+
-
Ceratitis capitata Wiedemann glycerone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 33500, SDS-PAGE Ceratitis capitata

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
31
-
-
Ceratitis capitata

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
20
-
between pH 6.0 and 9.0, stable for 1 h Ceratitis capitata
30
-
30 min stable, pH 6.6 Ceratitis capitata
45
-
pH 6.6, 5 min stable Ceratitis capitata
55
-
inactivation after 30 min Ceratitis capitata
60
-
inactivation after 5 min Ceratitis capitata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32
-
sn-glycerol 3-phosphate per subunit of 33500 Da Ceratitis capitata
188
-
dihydroxyacetone phosphate per subunit of 33500 Da Ceratitis capitata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
optimum pH for NADH oxidation around pH 6.6 Ceratitis capitata
10
-
optimum pH for NAD+ reduction about pH 10.0 Ceratitis capitata

pH Range

pH Minimum pH Maximum Comment Organism
5.2 7.8 about half-maximal activity between pH 5.2 and 7.8, reduction of dihydroxyacetone phosphate Ceratitis capitata
8 11 about half-maximal activity at pH 8.0 and 11.0, oxidation of L-glycerol-3-phosphate Ceratitis capitata

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
inactivation after 1 h at 20┬░C Ceratitis capitata
6 9 stable for 16 h at 20┬░C Ceratitis capitata
10
-
inactivation after 1 h at 20┬░C Ceratitis capitata

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ceratitis capitata
NADH
-
Ceratitis capitata