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Literature summary for 1.1.1.8 extracted from

  • Ostro, M.J.; Fondy, T.P.
    Isolation and characterization of multiple molecular forms of cytosolic NAD-linked glycerol-3-phosphate dehydrogenase from normal and neoplastic rabbit tissues (1977), J. Biol. Chem., 252, 5575-5583.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate 10 nM, strong inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information various Km-values of 4 isozymes of heart, muscle, liver and mammary gland Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Oryctolagus cuniculus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
x * 37500, isozyme from heart (II6.1), calculated by amino acid composition Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Oryctolagus cuniculus favoured reaction of heart isozyme II6.1 dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
several isozymes, distinct in isoelectric points Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
Brown Pearce carcinoma cell
-
Oryctolagus cuniculus
-
liver
-
Oryctolagus cuniculus
-
mammary gland
-
Oryctolagus cuniculus
-
muscle heart muscle Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
115
-
heart Oryctolagus cuniculus
180
-
mammary gland Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
4┬░C, 20% v/v, glycerol, purified stable Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Oryctolagus cuniculus dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ favoured reaction of heart isozyme II6.1 Oryctolagus cuniculus dihydroxyacetone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 37500, isozyme from heart (II6.1), calculated by amino acid composition Oryctolagus cuniculus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
48
-
t1/2: 2 min, heart isozyme II6.1 Oryctolagus cuniculus
48
-
t1/2: 2 min, brain isozyme I5.9 Oryctolagus cuniculus
48
-
t1/2: 15 min, muscle isozyme I6.5 Oryctolagus cuniculus
50
-
t1/2: 2 min in heart and 10 min in muscle, mammary gland and liver Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Oryctolagus cuniculus
additional information not NADP+ Oryctolagus cuniculus
NAD+
-
Oryctolagus cuniculus
NADH
-
Oryctolagus cuniculus