Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.8 extracted from

  • White, H.B.
    Glycerol phosphate dehydrogenase of chicken breast muscle (1975), Methods Enzymol., 41B, 245-249.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Gallus gallus

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification Gallus gallus
ammonium sulfate stabilizes Gallus gallus
NADH stabilizes preparations of low ionic strength Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
NADH
-
Gallus gallus
0.23
-
dihydroxyacetone phosphate
-
Gallus gallus
0.5
-
NAD+
-
Gallus gallus
2
-
glycerol 3-phosphate
-
Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Gallus gallus
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
muscle breast muscle Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
310
-
-
Gallus gallus

Storage Stability

Storage Stability Organism
crystallized, in ammonium sulfate stable for months Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Gallus gallus dihydroxyacetone phosphate + NADH + H+
-
r

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8 pH-rate profile for the reduction of dihydroxyacetone phosphate shows rather sharp optimum between pH 7.5 and 8.0 Gallus gallus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Gallus gallus
NAD+
-
Gallus gallus
NADH
-
Gallus gallus