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Literature summary for 1.1.1.8 extracted from

  • McGinnis, J.F.; DeVellis, J.
    Purification and characterization of rat brain glycerol phosphate dehydrogenase (1974), Biochim. Biophys. Acta, 364, 17-27.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification Rattus norvegicus
ammonium sulfate stabilizes Rattus norvegicus
bovine serum albumin stabilizes Rattus norvegicus
lyophilization inactivates Rattus norvegicus
phosphate buffer stabilizes Rattus norvegicus
repeated freezing and thawing results in rapid loss of activity, to some extent restorable at room temperature Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate reversible by dithiothreitol, 10 mM inhibits to 50% Rattus norvegicus
N-ethylmaleimide reversible by dithiothreitol, 5 mM inhibits more than 90% of the enzyme activity Rattus norvegicus
p-chloromercuribenzoate 10 nM, strong inhibition; reversible by dithiothreitol, 0.001 mM inhibits more than 90% of the enzyme activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADH less than 0.01 mM Rattus norvegicus
0.03
-
NAD+
-
Rattus norvegicus
0.17
-
dihydroxyacetone phosphate
-
Rattus norvegicus
0.3
-
alpha-glycerol phosphate
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
gel filtration Rattus norvegicus
75000
-
sucrose density gradient ultracentrifugation Rattus norvegicus
78000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Rattus norvegicus
-
dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ Rattus norvegicus Sprague-Dawley
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
ion-exchange chromatography combined with affinity elution, 2 isozymes that differ in charge by analytical PAGE Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
152.4
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Rattus norvegicus dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+
-
Rattus norvegicus Sprague-Dawley dihydroxyacetone phosphate + NADH + H+
-
r

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
45
-
at least 5 min stable Rattus norvegicus
50
-
rapid denaturation, prevented by 0.1 mg/ml bovine serum albumin, by over 20% ammonium sulfate or by 0.1 M phosphate buffer, pH 7.5 Rattus norvegicus
60
-
inactivation after 5 min Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
reduction of dihydroxyacetone phosphate, purified enzyme, decline in activity is more rapid at higher pH values, 7.3-7.5 crude homogenate of enzyme Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.2 about half-maximal activity at pH 6.5 and 8.2, reduction of dihydroxyacetone phosphate Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
more stable at alkaline pH, more stable in phosphate buffer than in Tris-glycine buffer Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus
NADH
-
Rattus norvegicus